8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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Details about selected cell
NeuroMorpho.Org ID :  NMO_143405
Cell Name :  10-6-15-ppkcd4gfp-uasshepha3x-bel30het-29c-lif_Series004
Archive Name :  Olesnicky
Species Name :  drosophila melanogaster
Strain :  ppkGal4,UAS-CD4-tdGFP (x) UASpAbp, UASshep3xHA (x) belneo30/TM3, Sb, Ser
Structural Domains :  Dendrites, No Soma, No Axon
Physical Integrity :  Dendrites Moderate
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  Not reported
Max Age :  Not reported
Gender :  Not reported
Min Weight :  Not reported
Max Weight :  Not reported
Development :  larval
Primary Brain Region :  peripheral nervous system
Secondary Brain Region :  abdominal
Tertiary Brain Region :  segment 4-6
Primary Cell Class :  sensory
Secondary Cell Class :  Multidendritic-dendritic arborization (DA)
Tertiary Cell Class :  class IV, ddaC
Original Format :  NeuronJ.ndf
Experiment Protocol :  in vivo
Experimental Condition :  Belle (Bel) 30 epistasis + shep overexpression
Staining Method :  green fluorescent protein, immunostaining
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  40x
Reconstruction Method :  NeuronJ
Date of Deposition :  2019-11-15
Date of Upload :  2020-11-05
Persistence Vector :  10-6-15-ppkcd4gfp-uasshepha3x-bel30het-29c-lif_Series004.pvec
Note :  This reconstruction was obtained from a transgene Shep overexpression. GAL4/UAS experiments for ddaC neuron dendrite defects were performed at 29 C to increase GAL4 efficiency. For Lexogen reads we inverted the strand flag of the first mate in the BAM files using in-house scripts to account for the reversed strandness of R1 when using the Lexogen SENSE library kit.

Reference Article
Related Article Reference :  Shep interacts with posttranscriptional regulators to control dendrite morphogenesis in sensory neurons.

Measurements
Soma Surface :  N/A
Number of Stems :  2
Number of Bifurcations :  232
Number of Branches :  466
Overall Width :  1006.59 μm
Overall Height :  961.19 μm
Overall Depth :  0 μm
Average Diameter :  0.25 μm
Total Length :  16447.3 μm
Total Surface :  12917.7 μm2
Total Volume :  807.36 μm3
Max Euclidean Distance :  995.63 μm
Max Path Distance :  1563.07 μm
Max Branch Order :  25
Average Contraction :  0.96
Total Fragmentation :  3199
Partition Asymmetry :  0.61
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  82.71°
Average Bifurcation Angle Remote :  92.79°
Fractal Dimension :  1.01

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