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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_136311 |
| Cell Name : | siSiah2_Bic48h_290917_1-0001 |
| Archive Name : | Soriano |
| Species Name : | rat |
| Strain : | Sprague-Dawley |
| Structural Domains : | Dendrites, Soma, Axon |
| Physical Integrity : | Dendrites & Axon Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 19.5 days
|
| Max Age : | 20.5 days
|
| Gender : |
Not reported
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | Not reported |
| Tertiary Cell Class : | Not reported |
| Original Format : | Simple Neurite Tracer.traces |
| Experiment Protocol : | culture |
| Experimental Condition : | Siah E3 ubiquitin-protein ligase 2 (SIAH2)-targeting small interfering RNAs (siRNAs), bicuculline (Bic) plus 4-amino pyridine (4-AP) |
| Staining Method : | green fluorescent protein, immunostaining |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 4x |
| Reconstruction Method : | Simple Neurite Tracer |
| Date of Deposition : | 2020-03-24 |
| Date of Upload : | 2020-08-10 |
| Persistence Vector : | siSiah2_Bic48h_290917_1-0001.pvec |
| Note : | For knockdown experiments, 25 ng of rat Siah2 (L-089773-02) were used. Neurons were stimulated for 48 h with Bic + 4-AP after transfection. |
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| Reference Article |
| Related Article Reference : | Synaptic activity-induced glycolysis facilitates membrane lipid provision and neurite outgrowth. |
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| Measurements |
| Soma Surface : |
503.22 μm2 |
| Number of Stems : |
4 |
| Number of Bifurcations : |
79 |
| Number of Branches : |
162 |
| Overall Width : |
4346.18 μm |
| Overall Height : |
2525.98 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
24762.8 μm |
| Total Surface : |
19448.6 μm2 |
| Total Volume : |
1215.54 μm3 |
| Max Euclidean Distance : |
2937.32 μm |
| Max Path Distance : |
4621.42 μm |
| Max Branch Order : |
27 |
| Average Contraction : |
0.94 |
| Total Fragmentation : |
12981 |
| Partition Asymmetry : |
0.78 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
35.7° |
| Average Bifurcation Angle Remote : |
48.75° |
| Fractal Dimension : |
1.02 |
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