8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_287110
Cell Name :  d5_20X__0009_RFP
Archive Name :  Firestein
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  18.0 days
Max Age :  18.0 days
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  embryonic
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  Not reported
Tertiary Cell Class :  Not reported
Original Format :  NeuronJ.swc
Experiment Protocol :  culture
Experimental Condition :  Membrane protein alpha-Klotho (mKl) overexpression
Staining Method :  red fluorescent protein
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  20x
Reconstruction Method :  NeuronJ
Date of Deposition :  2024-07-25
Date of Upload :  2024-10-01
Persistence Vector :  d5_20X__0009_RFP.pvec
Note :  Hippocampal cultures were co-transfected at DIV 7 with a construct expressing mRFP under a CMV promoter and a plasmid containing mKl-IRES-GFP (VectorBuilder, VB211220-1173gzn).

Reference Article
Related Article Reference :  Overexpression of α-Klotho isoforms promotes distinct Effects on BDNF-Induced Alterations in Dendritic Morphology.

Measurements
Soma Surface :  188.04 μm2
Number of Stems :  9
Number of Bifurcations :  17
Number of Branches :  43
Overall Width :  56.89 μm
Overall Height :  108.37 μm
Overall Depth :  0 μm
Average Diameter :  0.3 μm
Total Length :  680.2 μm
Total Surface :  641.07 μm2
Total Volume :  48.08 μm3
Max Euclidean Distance :  82.17 μm
Max Path Distance :  94.56 μm
Max Branch Order :  6
Average Contraction :  0.94
Total Fragmentation :  1184
Partition Asymmetry :  0.65
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  44.68°
Average Bifurcation Angle Remote :  58.67°
Fractal Dimension :  1.02

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