8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_287068
Cell Name :  d4_20X__0012_RFP
Archive Name :  Firestein
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  18.0 days
Max Age :  18.0 days
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  embryonic
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  Not reported
Tertiary Cell Class :  Not reported
Original Format :  NeuronJ.swc
Experiment Protocol :  culture
Experimental Condition :  Truncated secreted alpha-Klotho (sKl) overexpression
Staining Method :  red fluorescent protein
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  20x
Reconstruction Method :  NeuronJ
Date of Deposition :  2024-07-25
Date of Upload :  2024-10-01
Persistence Vector :  d4_20X__0012_RFP.pvec
Note :  Hippocampal cultures were co-transfected at DIV 7 with a construct expressing mRFP under a CMV promoter and a plasmid containing sKl-IRES-GFP (VectorBuilder, VB211220-1169ryc).

Reference Article
Related Article Reference :  Overexpression of α-Klotho isoforms promotes distinct Effects on BDNF-Induced Alterations in Dendritic Morphology.

Measurements
Soma Surface :  59.05 μm2
Number of Stems :  5
Number of Bifurcations :  11
Number of Branches :  27
Overall Width :  47.38 μm
Overall Height :  46.74 μm
Overall Depth :  0 μm
Average Diameter :  0.3 μm
Total Length :  243.19 μm
Total Surface :  229.21 μm2
Total Volume :  17.19 μm3
Max Euclidean Distance :  46.93 μm
Max Path Distance :  52.64 μm
Max Branch Order :  5
Average Contraction :  0.95
Total Fragmentation :  425
Partition Asymmetry :  0.45
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  51.13°
Average Bifurcation Angle Remote :  72.7°
Fractal Dimension :  1.02

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