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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_287133 |
| Cell Name : | d4_20X_12_0012_RFP |
| Archive Name : | Firestein |
| Species Name : | rat |
| Strain : | Sprague-Dawley |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 18.0 days
|
| Max Age : | 18.0 days
|
| Gender : |
Male/Female
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| Min Weight : | Not reported
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| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | Not reported |
| Tertiary Cell Class : | Not reported |
| Original Format : | NeuronJ.swc |
| Experiment Protocol : | culture |
| Experimental Condition : | Brain-derived neurotrophic factor (BDNF) + Membrane protein alpha-Klotho (mKl) overexpression |
| Staining Method : | red fluorescent protein |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 20x |
| Reconstruction Method : | NeuronJ |
| Date of Deposition : | 2024-07-25 |
| Date of Upload : | 2024-10-01 |
| Persistence Vector : | d4_20X_12_0012_RFP.pvec |
| Note : | Hippocampal cultures were co-transfected at DIV 7 with a construct expressing mRFP under a CMV promoter and a plasmid containing mKl-IRES-GFP (VectorBuilder, VB211220-1173gzn). Hippocampal cultures were treated with 25 ng/mL of BDNF for two different durations: a 5 min treatment at DIV 11, and a 72 h treatment from DIV 11 until DIV 14. |
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| Reference Article |
| Related Article Reference : | Overexpression of α-Klotho isoforms promotes distinct Effects on BDNF-Induced Alterations in Dendritic Morphology. |
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| Measurements |
| Soma Surface : |
137.32 μm2 |
| Number of Stems : |
8 |
| Number of Bifurcations : |
21 |
| Number of Branches : |
50 |
| Overall Width : |
43.18 μm |
| Overall Height : |
54.58 μm |
| Overall Depth : |
0.08 μm |
| Average Diameter : |
0.3 μm |
| Total Length : |
345.5 μm |
| Total Surface : |
325.63 μm2 |
| Total Volume : |
24.42 μm3 |
| Max Euclidean Distance : |
52.6 μm |
| Max Path Distance : |
55.29 μm |
| Max Branch Order : |
10 |
| Average Contraction : |
0.96 |
| Total Fragmentation : |
584 |
| Partition Asymmetry : |
0.61 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
46.31° |
| Average Bifurcation Angle Remote : |
47.55° |
| Fractal Dimension : |
1.01 |
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