8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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Details about selected cell
NeuroMorpho.Org ID :  NMO_245378
Cell Name :  cv3n45_2
Archive Name :  Guizzetti
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Neurites, Soma
Physical Integrity :  Neurites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  1.0 day
Max Age :  1.0 day
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  neonatal
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Not reported
Original Format :  Neurolucida.dat
Experiment Protocol :  culture
Experimental Condition :  siPlat
Staining Method :  immunostaining
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  40x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-05-05
Date of Upload :  2022-11-07
Persistence Vector :  cv3n45_2.pvec
Note :  Two days after sub-culture, astrocytes were transfected in DMEM/0.1% BSA without antibiotics supplemented with 10 microliters/well, containing 1 nM Stealth siRNA targeting the rat Plat. This reconstruction was obtained 48 h after transfection.

Reference Article
Related Article Reference :  Astrocyte tissue plasminogen activator expression during brain development and its role in pyramidal neuron neurite outgrowth.

Measurements
Soma Surface :  705.76 μm2
Number of Stems :  4
Number of Bifurcations :  6
Number of Branches :  16
Overall Width :  54.23 μm
Overall Height :  43.12 μm
Overall Depth :  0.9 μm
Average Diameter :  1 μm
Total Length :  232.51 μm
Total Surface :  850 μm2
Total Volume :  353.99 μm3
Max Euclidean Distance :  31.15 μm
Max Path Distance :  39.36 μm
Max Branch Order :  2
Average Contraction :  0.91
Total Fragmentation :  143
Partition Asymmetry :  0.17
Average Rall's Ratio :  1.88
Average Bifurcation Angle Local :  62.77°
Average Bifurcation Angle Remote :  54.36°
Fractal Dimension :  1.05

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