8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_250053
Cell Name :  c3_13
Archive Name :  Garcia-Hernandez_DeSantis
Species Name :  rat
Strain :  Wistar
Structural Domains :  Processes, Soma
Physical Integrity :  Processes Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  2.0 months
Max Age :  3.0 months
Gender :  Male
Min Weight :  300 grams
Max Weight :  400 grams
Development :  adult
Primary Brain Region :  hippocampus
Secondary Brain Region :  dentate gyrus
Tertiary Brain Region :  granule layer
Primary Cell Class :  Glia
Secondary Cell Class :  microglia
Tertiary Cell Class :  Iba1-positive
Original Format :  Neurolucida.dat
Experiment Protocol :  in vivo
Experimental Condition :  lipopolysaccharide injection
Staining Method :  immunostaining
Slicing Direction :  coronal
Slice Thickness :  50  μm
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  20x , 40x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-11-02
Date of Upload :  2022-12-07
Persistence Vector :  c3_13.pvec
Note :  Glia activation was achieved by intracranial injection in the dorsal hippocampus (coordinates: bregma, -3.8 mm; superior-inferior, 3.0 mm; 2 mm from midline in the left hemisphere) of 2 microliters of saline and LPS at a concentration of 2.5 microgram/microliter. This reconstruction was obtained 15 days post-injection.

Reference Article
Related Article Reference :  Mapping microglia and astrocyte activation in vivo using diffusion MRI.

Measurements
Soma Surface :  150.3 μm2
Number of Stems :  7
Number of Bifurcations :  28
Number of Branches :  63
Overall Width :  53.7 μm
Overall Height :  34.07 μm
Overall Depth :  12.39 μm
Average Diameter :  0.25 μm
Total Length :  418.59 μm
Total Surface :  328.76 μm2
Total Volume :  20.55 μm3
Max Euclidean Distance :  41.02 μm
Max Path Distance :  46.83 μm
Max Branch Order :  7
Average Contraction :  0.95
Total Fragmentation :  159
Partition Asymmetry :  0.57
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  88.66°
Average Bifurcation Angle Remote :  78.38°
Fractal Dimension :  1.03

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