8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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Details about selected cell
NeuroMorpho.Org ID :  NMO_287079
Cell Name :  b5_20X_7_0007_RFP
Archive Name :  Firestein
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  18.0 days
Max Age :  18.0 days
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  embryonic
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  Not reported
Tertiary Cell Class :  Not reported
Original Format :  NeuronJ.swc
Experiment Protocol :  culture
Experimental Condition :  Truncated secreted alpha-Klotho (sKl) overexpression
Staining Method :  red fluorescent protein
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  20x
Reconstruction Method :  NeuronJ
Date of Deposition :  2024-07-25
Date of Upload :  2024-10-01
Persistence Vector :  b5_20X_7_0007_RFP.pvec
Note :  Hippocampal cultures were co-transfected at DIV 7 with a construct expressing mRFP under a CMV promoter and a plasmid containing sKl-IRES-GFP (VectorBuilder, VB211220-1169ryc).

Reference Article
Related Article Reference :  Overexpression of α-Klotho isoforms promotes distinct Effects on BDNF-Induced Alterations in Dendritic Morphology.

Measurements
Soma Surface :  223.27 μm2
Number of Stems :  8
Number of Bifurcations :  22
Number of Branches :  52
Overall Width :  49.9 μm
Overall Height :  126.16 μm
Overall Depth :  0 μm
Average Diameter :  0.3 μm
Total Length :  660.58 μm
Total Surface :  622.58 μm2
Total Volume :  46.69 μm3
Max Euclidean Distance :  97.03 μm
Max Path Distance :  109.84 μm
Max Branch Order :  8
Average Contraction :  0.92
Total Fragmentation :  1161
Partition Asymmetry :  0.55
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  59.7°
Average Bifurcation Angle Remote :  50.25°
Fractal Dimension :  1.03

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