8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
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Log File (Original)



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NeuroMorpho.Org ID :  NMO_171005
Cell Name :  Zeb2_cKO_122-B2-3-1mm-R-2a
Archive Name :  Vivinetto_Cave
Species Name :  mouse
Strain :  Gfap-creERT2/Zeb2fl/fl
Structural Domains :  Processes, No Soma
Physical Integrity :  Processes Moderate
Morphological Attributes :  Diameter, 3D, Angles
Min Age :  3.0 months
Max Age :  4.0 months
Gender :  Male/Female
Min Weight :  25 grams
Max Weight :  25 grams
Development :  adult
Primary Brain Region :  spinal cord
Secondary Brain Region :  thoracic
Tertiary Brain Region :  rigth
Primary Cell Class :  Glia
Secondary Cell Class :  astrocyte
Tertiary Cell Class :  GFAP-positive
Original Format :  Neurolucida.traces
Experiment Protocol :  in vitro
Experimental Condition :  Spinal cord T9 lesion + Zeb2c knockout
Staining Method :  immunostaining
Slicing Direction :  coronal
Slice Thickness :  20  μm
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  10x
Reconstruction Method :  Neurolucida
Date of Deposition :  2020-08-08
Date of Upload :  2021-12-17
Persistence Vector :  Zeb2_cKO_122-B2-3-1mm-R-2a.pvec
Note :  On the day of injury, mice were anesthetized with isoflurane (2.5% before performing a laminectomy to remove the dorsal process of thoracic vertebrae 9 (T9) and expose the spinal cord. The lateral processes of T8 and T10 were clamped and a 50 kDyn injury was induced using the Infinite Horizon Impactor. This reconstruction was obtained at a 1mm distance from the lesion boundary.

Reference Article
Related Article Reference :  Zeb2 Is a Regulator of Astrogliosis and Functional Recovery after CNS Injury.

Measurements
Soma Surface :  N/A
Number of Stems :  2
Number of Bifurcations :  78
Number of Branches :  158
Overall Width :  53.27 μm
Overall Height :  43.67 μm
Overall Depth :  7.18 μm
Average Diameter :  0.25 μm
Total Length :  797.08 μm
Total Surface :  641.98 μm2
Total Volume :  43.23 μm3
Max Euclidean Distance :  60.6 μm
Max Path Distance :  142.28 μm
Max Branch Order :  41
Average Contraction :  0.88
Total Fragmentation :  3315
Partition Asymmetry :  0.64
Average Rall's Ratio :  1.95
Average Bifurcation Angle Local :  90.45°
Average Bifurcation Angle Remote :  85.87°
Fractal Dimension :  1.09

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