8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
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Log File (Original)



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NeuroMorpho.Org ID :  NMO_171084
Cell Name :  Zeb2_cKO_122-B2-1-3mm-L-1
Archive Name :  Vivinetto_Cave
Species Name :  mouse
Strain :  Gfap-creERT2/Zeb2fl/fl
Structural Domains :  Processes, No Soma
Physical Integrity :  Processes Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  3.0 months
Max Age :  4.0 months
Gender :  Male/Female
Min Weight :  25 grams
Max Weight :  25 grams
Development :  adult
Primary Brain Region :  spinal cord
Secondary Brain Region :  thoracic
Tertiary Brain Region :  left
Primary Cell Class :  Glia
Secondary Cell Class :  astrocyte
Tertiary Cell Class :  GFAP-positive
Original Format :  Neurolucida.traces
Experiment Protocol :  in vitro
Experimental Condition :  Spinal cord T9 lesion + Zeb2c knockout
Staining Method :  immunostaining
Slicing Direction :  coronal
Slice Thickness :  20  μm
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  10x
Reconstruction Method :  Neurolucida
Date of Deposition :  2020-08-08
Date of Upload :  2021-12-17
Persistence Vector :  Zeb2_cKO_122-B2-1-3mm-L-1.pvec
Note :  On the day of injury, mice were anesthetized with isoflurane (2.5% before performing a laminectomy to remove the dorsal process of thoracic vertebrae 9 (T9) and expose the spinal cord. The lateral processes of T8 and T10 were clamped and a 50 kDyn injury was induced using the Infinite Horizon Impactor. This reconstruction was obtained at a 3mm distance from the lesion boundary.

Reference Article
Related Article Reference :  Zeb2 Is a Regulator of Astrogliosis and Functional Recovery after CNS Injury.

Measurements
Soma Surface :  N/A
Number of Stems :  1
Number of Bifurcations :  78
Number of Branches :  157
Overall Width :  62.25 μm
Overall Height :  44.98 μm
Overall Depth :  6.89 μm
Average Diameter :  0.25 μm
Total Length :  881.86 μm
Total Surface :  692.61 μm2
Total Volume :  43.29 μm3
Max Euclidean Distance :  42.67 μm
Max Path Distance :  60.53 μm
Max Branch Order :  23
Average Contraction :  0.88
Total Fragmentation :  3776
Partition Asymmetry :  0.66
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  61.67°
Average Bifurcation Angle Remote :  72.41°
Fractal Dimension :  1.07

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