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Details about selected cell |
NeuroMorpho.Org ID : | NMO_191585 |
Cell Name : | WvHMM12_18DIV_cs_93_c__7_PSD95_GFP |
Archive Name : | Bastian |
Species Name : | mouse |
Strain : | CD1 |
Structural Domains : | Dendrites, Soma, No Axon |
Physical Integrity : | Dendrites Complete |
Morphological Attributes : | No Diameter, 2D, Angles |
Min Age : | 16.0 days
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Max Age : | 16.0 days
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Gender : |
Male/Female
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Min Weight : | Not reported
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Max Weight : | Not reported
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Development : | embryonic |
Primary Brain Region : | hippocampus |
Secondary Brain Region : | Not reported |
Tertiary Brain Region : | Not reported |
Primary Cell Class : | principal cell |
Secondary Cell Class : | Pyramidal-like |
Tertiary Cell Class : | Microtubule-associated protein 2 (MAP2)-positive |
Original Format : | Simple Neurite Tracer.traces |
Experiment Protocol : | culture |
Experimental Condition : | Iron repletion + choline |
Staining Method : | green fluorescent protein |
Slicing Direction : | Not applicable |
Slice Thickness : | Not applicable
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Tissue Shrinkage : |
Not reported
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Objective Type : | dry |
Magnification : | 20x |
Reconstruction Method : | Simple Neurite Tracer |
Date of Deposition : | 2022-06-09 |
Date of Upload : | 2022-06-24 |
Persistence Vector : | WvHMM12_18DIV_cs_93_c__7_PSD95_GFP.pvec |
Note : | Neuronal iron deficiency was created beginning at 3 DIV by treating cultures with 9 micromolar deferoxamine. At 11 DIV, for the formerly iron-deficient (FID), iron repletion was accomplished by removing all culture medium from a subset of DFO-treated cultures and immediately replacing it with a half-volume of the medium that was removed from iron sufficient (IS) cultures and supplemented with an additional 30 micromolar choline chloride. |
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Reference Article |
Related Article Reference : | Choline Supplementation Partially Restores Dendrite Structural Complexity in Developing Iron-Deficient Mouse Hippocampal Neurons. |
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Measurements |
Soma Surface : |
313.52 μm2 |
Number of Stems : |
5 |
Number of Bifurcations : |
44 |
Number of Branches : |
93 |
Overall Width : |
244.52 μm |
Overall Height : |
327.39 μm |
Overall Depth : |
0 μm |
Average Diameter : |
0.25 μm |
Total Length : |
2116.59 μm |
Total Surface : |
1662.36 μm2 |
Total Volume : |
103.9 μm3 |
Max Euclidean Distance : |
185.9 μm |
Max Path Distance : |
273.83 μm |
Max Branch Order : |
14 |
Average Contraction : |
0.91 |
Total Fragmentation : |
5628 |
Partition Asymmetry : |
0.7 |
Average Rall's Ratio : |
2 |
Average Bifurcation Angle Local : |
35.64° |
Average Bifurcation Angle Remote : |
62.74° |
Fractal Dimension : |
1.03 |
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