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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_191516 |
| Cell Name : | WvHMM12_18DIV_cs_93_c__6_PSD95_GFP |
| Archive Name : | Bastian |
| Species Name : | mouse |
| Strain : | CD1 |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 16.0 days
|
| Max Age : | 16.0 days
|
| Gender : |
Male/Female
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| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | Pyramidal-like |
| Tertiary Cell Class : | Microtubule-associated protein 2 (MAP2)-positive |
| Original Format : | Simple Neurite Tracer.traces |
| Experiment Protocol : | culture |
| Experimental Condition : | Iron repletion + choline |
| Staining Method : | green fluorescent protein |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 20x |
| Reconstruction Method : | Simple Neurite Tracer |
| Date of Deposition : | 2022-06-09 |
| Date of Upload : | 2022-06-24 |
| Persistence Vector : | WvHMM12_18DIV_cs_93_c__6_PSD95_GFP.pvec |
| Note : | Neuronal iron deficiency was created beginning at 3 DIV by treating cultures with 9 micromolar deferoxamine. At 11 DIV, for the formerly iron-deficient (FID), iron repletion was accomplished by removing all culture medium from a subset of DFO-treated cultures and immediately replacing it with a half-volume of the medium that was removed from iron sufficient (IS) cultures and supplemented with an additional 30 micromolar choline chloride. |
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| Reference Article |
| Related Article Reference : | Choline Supplementation Partially Restores Dendrite Structural Complexity in Developing Iron-Deficient Mouse Hippocampal Neurons. |
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| Measurements |
| Soma Surface : |
312.91 μm2 |
| Number of Stems : |
6 |
| Number of Bifurcations : |
72 |
| Number of Branches : |
150 |
| Overall Width : |
295.01 μm |
| Overall Height : |
446.15 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
2816.6 μm |
| Total Surface : |
2212.15 μm2 |
| Total Volume : |
138.26 μm3 |
| Max Euclidean Distance : |
259.16 μm |
| Max Path Distance : |
426.4 μm |
| Max Branch Order : |
18 |
| Average Contraction : |
0.92 |
| Total Fragmentation : |
7477 |
| Partition Asymmetry : |
0.64 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
56.57° |
| Average Bifurcation Angle Remote : |
69.22° |
| Fractal Dimension : |
1.03 |
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0
