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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_160358 |
| Cell Name : | Snap-25266 |
| Archive Name : | Carriba |
| Species Name : | mouse |
| Strain : | B6129P2-Cd40tm1Kik/J (002928 Jax) x CD1 |
| Structural Domains : | Neurites, No Soma |
| Physical Integrity : | Neurites Moderate |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 14.0 days
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| Max Age : | 14.0 days
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| Gender : |
Male/Female
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| Min Weight : | Not reported
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| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | basal ganglia |
| Secondary Brain Region : | striatum |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | medium spiny |
| Tertiary Cell Class : | GABAergic, dopamine and cyclic AMP-regulated protein (DARPP-32)-positive |
| Original Format : | Simple Neurite Tracer.traces |
| Experiment Protocol : | in vitro |
| Experimental Condition : | CD40-Fc + Go6983 + flavonoid fisetin (Fis) |
| Staining Method : | immunostaining |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
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| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 10x |
| Reconstruction Method : | Simple Neurite Tracer |
| Date of Deposition : | 2021-07-19 |
| Date of Upload : | 2021-07-28 |
| Persistence Vector : | Snap-25266.pvec |
| Note : | These mice were back-crossed into a CD1 background. Cd40+/- mice were crossed to generate Cd40-/- mice from which cultures were established. The cultures were treated with the following reagents as indicated in the text: CD40-Fc (1 microgram/mL), Fisetin (1 micromolar), and Go 6983 (500 nM). For the analysis of neurite morphology, treatments were performed the next day after seeding the neurons. Neuronal morphology was retrieved after 10 days. |
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| Reference Article |
| Related Article Reference : | Signalling Pathways Mediating the Effects of CD40-Activated CD40L Reverse Signalling on Inhibitory Medium Spiny Neuron Neurite Growth |
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| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
2 |
| Number of Bifurcations : |
19 |
| Number of Branches : |
40 |
| Overall Width : |
236.68 μm |
| Overall Height : |
171.66 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
1052.03 μm |
| Total Surface : |
826.26 μm2 |
| Total Volume : |
51.64 μm3 |
| Max Euclidean Distance : |
209.69 μm |
| Max Path Distance : |
393.5 μm |
| Max Branch Order : |
12 |
| Average Contraction : |
0.9 |
| Total Fragmentation : |
3225 |
| Partition Asymmetry : |
0.65 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
94.81° |
| Average Bifurcation Angle Remote : |
97.91° |
| Fractal Dimension : |
1.04 |
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0
