8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_161918
Cell Name :  Shisa6_4_Trace
Archive Name :  Ferguson
Species Name :  mouse
Strain :  Ai6; D1-Cre
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  70.0 days
Max Age :  84.0 days
Gender :  Male
Min Weight :  25 grams
Max Weight :  30 grams
Development :  adult
Primary Brain Region :  basal ganglia
Secondary Brain Region :  ventral striatum
Tertiary Brain Region :  nucleus accumbens
Primary Cell Class :  principal cell
Secondary Cell Class :  medium spiny
Tertiary Cell Class :  D1-type dopamine receptor-expressing
Original Format :  Neurolucida.dat
Experiment Protocol :  in vitro
Experimental Condition :  Shisa6
Staining Method :  biocytin
Slicing Direction :  coronal
Slice Thickness :  350  μm
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  20x
Reconstruction Method :  Neurolucida
Date of Deposition :  2021-08-02
Date of Upload :  2021-08-11
Persistence Vector :  Shisa6_4_Trace.pvec
Note :  Mice at the age of 7 weeks were anesthetized with a ketamine/xylazine mixture (ketamine 100 mg/kg and xylazine 10 mg/kg) and prepared for stereotaxic surgery. Thirty-three gauge syringe needles were used to bilaterally infuse 0.5 microliters of AAV-DIO-Shisa6-mCherry into the NAc (AP: 1.5, ML: 1.5, DV: 4.4, angle: 10 degrees).

Reference Article
Related Article Reference :  Shisa6 mediates cell-type specific regulation of depression in the nucleus accumbens.

Measurements
Soma Surface :  354.73 μm2
Number of Stems :  6
Number of Bifurcations :  11
Number of Branches :  28
Overall Width :  202.8 μm
Overall Height :  292.23 μm
Overall Depth :  39.98 μm
Average Diameter :  1.31 μm
Total Length :  2066.35 μm
Total Surface :  8496.77 μm2
Total Volume :  2780.98 μm3
Max Euclidean Distance :  216.94 μm
Max Path Distance :  255.51 μm
Max Branch Order :  3
Average Contraction :  0.8
Total Fragmentation :  704
Partition Asymmetry :  0.27
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  77.45°
Average Bifurcation Angle Remote :  89.97°
Fractal Dimension :  1.05

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