8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_161903
Cell Name :  Shisa6_3_Trace
Archive Name :  Ferguson
Species Name :  mouse
Strain :  Ai6; D1-Cre
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  70.0 days
Max Age :  84.0 days
Gender :  Male
Min Weight :  25 grams
Max Weight :  30 grams
Development :  adult
Primary Brain Region :  basal ganglia
Secondary Brain Region :  ventral striatum
Tertiary Brain Region :  nucleus accumbens
Primary Cell Class :  principal cell
Secondary Cell Class :  medium spiny
Tertiary Cell Class :  D1-type dopamine receptor-expressing
Original Format :  Neurolucida.dat
Experiment Protocol :  in vitro
Experimental Condition :  Shisa6
Staining Method :  biocytin
Slicing Direction :  coronal
Slice Thickness :  350  μm
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  20x
Reconstruction Method :  Neurolucida
Date of Deposition :  2021-08-02
Date of Upload :  2021-08-11
Persistence Vector :  Shisa6_3_Trace.pvec
Note :  Mice at the age of 7 weeks were anesthetized with a ketamine/xylazine mixture (ketamine 100 mg/kg and xylazine 10 mg/kg) and prepared for stereotaxic surgery. Thirty-three gauge syringe needles were used to bilaterally infuse 0.5 microliters of AAV-DIO-Shisa6-mCherry into the NAc (AP: 1.5, ML: 1.5, DV: 4.4, angle: 10 degrees).

Reference Article
Related Article Reference :  Shisa6 mediates cell-type specific regulation of depression in the nucleus accumbens.

Measurements
Soma Surface :  286.47 μm2
Number of Stems :  5
Number of Bifurcations :  9
Number of Branches :  23
Overall Width :  165.47 μm
Overall Height :  255.33 μm
Overall Depth :  27.91 μm
Average Diameter :  0.19 μm
Total Length :  1256.81 μm
Total Surface :  750.19 μm2
Total Volume :  35.63 μm3
Max Euclidean Distance :  153.66 μm
Max Path Distance :  198.52 μm
Max Branch Order :  3
Average Contraction :  0.85
Total Fragmentation :  535
Partition Asymmetry :  0.33
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  62.1°
Average Bifurcation Angle Remote :  56.95°
Fractal Dimension :  1.04

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