8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_183930
Cell Name :  Sept30IR3h-MM
Archive Name :  Medalla
Species Name :  monkey
Strain :  Rhesus
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  6.0 years
Max Age :  9.0 years
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  neocortex
Secondary Brain Region :  anterior cingulate
Tertiary Brain Region :  layer 3
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  dorsal premotor cortex (PMd)-projecting
Original Format :  Neuronstudio.swc
Experiment Protocol :  in vitro
Experimental Condition :  Control
Staining Method :  Biocytin, Streptavidin-Alexa 405
Slicing Direction :  coronal
Slice Thickness :  300  μm
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  40x
Reconstruction Method :  NeuronStudio
Date of Deposition :  2022-04-29
Date of Upload :  2022-05-25
Persistence Vector :  Sept30IR3h-MM.pvec
Note :  We injected a combination of dextran amine tracers, fluoroemerald and fluororuby. We used a cocktail of the 3KDA and 10kDA isoforms of dextran amines to ensure enhanced bidirectional retrograde and anterograde transport. For PMd injections, the tracer was delivered in 2-3 penetrations (spaced 0.5 mm apart) at a depth of 1.2-1.6 mm below the pial surface of the rostral PMd, to deliver a total of 3-8 microliters per injection site. For each injection, the needle was left in situ for 5-10 mins to allow for diffusion and prevent upward suction of the dye upon retraction of the needle.

Reference Article
Related Article Reference :  Layer-specific pyramidal neuron properties underlie diverse anterior cingulate cortical motor and limbic networks.

Measurements
Soma Surface :  1132.9 μm2
Number of Stems :  9
Number of Bifurcations :  47
Number of Branches :  103
Overall Width :  369.94 μm
Overall Height :  713.84 μm
Overall Depth :  86.08 μm
Average Diameter :  0.65 μm
Total Length :  8365.45 μm
Total Surface :  20431.2 μm2
Total Volume :  5551.52 μm3
Max Euclidean Distance :  555.98 μm
Max Path Distance :  652.99 μm
Max Branch Order :  12
Average Contraction :  0.89
Total Fragmentation :  12070
Partition Asymmetry :  0.42
Average Rall's Ratio :  3.24
Average Bifurcation Angle Local :  76.28°
Average Bifurcation Angle Remote :  57.81°
Fractal Dimension :  1.03

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