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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_182179 |
| Cell Name : | SCGN-01 |
| Archive Name : | Raju_Oldham |
| Species Name : | mouse |
| Strain : | Vip-Cre; R26-Ai14 |
| Structural Domains : | Neurites, Soma |
| Physical Integrity : | Neurites Complete |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 50.0 days
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| Max Age : | 50.0 days
|
| Gender : |
Male/Female
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| Min Weight : | Not reported
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| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | occipital |
| Tertiary Brain Region : | primary visual |
| Primary Cell Class : | interneuron |
| Secondary Cell Class : | GABAergic |
| Tertiary Cell Class : | Vasoactive Intestinal Peptide (VIP)-positive |
| Original Format : | Neurolucida.dat |
| Experiment Protocol : | in vivo |
| Experimental Condition : | calcium-binding protein secretagogin (SCGN) expression |
| Staining Method : | green fluorescent protein |
| Slicing Direction : | coronal |
| Slice Thickness : | 300
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | Not reported
|
| Reconstruction Method : | Neurolucida |
| Date of Deposition : | 2022-03-30 |
| Date of Upload : | 2022-04-20 |
| Persistence Vector : | SCGN-01.pvec |
| Note : | The ventricular and subventricular zones of E14.5 CGE (Vip-Cre and R26-Ai14) were dissected from donor embryos. Dissociated cells were infected with lenti-Gfp-SCGN virus and injected cells into the cortex. Cells were visualized by GFP and tdTomato and imaged by confocal microscopy. |
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| Reference Article |
| Related Article Reference : | Secretagogin is Expressed by Developing Neocortical GABAergic Neurons in Humans but not Mice and Increases Neurite Arbor Size and Complexity. |
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| Measurements |
| Soma Surface : |
630.02 μm2 |
| Number of Stems : |
4 |
| Number of Bifurcations : |
41 |
| Number of Branches : |
86 |
| Overall Width : |
577.2 μm |
| Overall Height : |
487.7 μm |
| Overall Depth : |
55.84 μm |
| Average Diameter : |
0.32 μm |
| Total Length : |
6819.57 μm |
| Total Surface : |
6964.46 μm2 |
| Total Volume : |
593.13 μm3 |
| Max Euclidean Distance : |
417.16 μm |
| Max Path Distance : |
964.33 μm |
| Max Branch Order : |
17 |
| Average Contraction : |
0.93 |
| Total Fragmentation : |
906 |
| Partition Asymmetry : |
0.73 |
| Average Rall's Ratio : |
2.12 |
| Average Bifurcation Angle Local : |
101.54° |
| Average Bifurcation Angle Remote : |
71.85° |
| Fractal Dimension : |
1.02 |
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