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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_250047 |
| Cell Name : | R01_594_Control_M32 |
| Archive Name : | Garcia-Hernandez_DeSantis |
| Species Name : | rat |
| Strain : | Wistar |
| Structural Domains : | Processes, Soma |
| Physical Integrity : | Processes Moderate |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 2.0 months
|
| Max Age : | 3.0 months
|
| Gender : |
Male
|
| Min Weight : | 300 grams
|
| Max Weight : | 400 grams
|
| Development : | adult |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | dentate gyrus |
| Tertiary Brain Region : | granule layer |
| Primary Cell Class : | Glia |
| Secondary Cell Class : | astrocyte |
| Tertiary Cell Class : | GFAP-positive |
| Original Format : | Neurolucida.dat |
| Experiment Protocol : | in vivo |
| Experimental Condition : | microglia depletion (PLX5622) + lipopolysaccharide injection |
| Staining Method : | immunostaining |
| Slicing Direction : | coronal |
| Slice Thickness : | 50
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 20x , 40x |
| Reconstruction Method : | Neurolucida |
| Date of Deposition : | 2022-11-02 |
| Date of Upload : | 2022-12-07 |
| Persistence Vector : | R01_594_Control_M32.pvec |
| Note : | Glia activation was achieved by intracranial injection in the dorsal hippocampus (coordinates: bregma, -3.8 mm; superior-inferior, 3.0 mm; 2 mm from midline in the left hemisphere) of 2 microliters of saline and LPS at a concentration of 2.5 microgram/microliter. Microglia depletion was achieved by administering the CSF1R inhibitor PLX5622. This reconstruction was obtained 24 hours post-injection. |
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| Reference Article |
| Related Article Reference : | Mapping microglia and astrocyte activation in vivo using diffusion MRI. |
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| Measurements |
| Soma Surface : |
67.88 μm2 |
| Number of Stems : |
7 |
| Number of Bifurcations : |
39 |
| Number of Branches : |
85 |
| Overall Width : |
47.37 μm |
| Overall Height : |
50.63 μm |
| Overall Depth : |
1.04 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
570.03 μm |
| Total Surface : |
447.7 μm2 |
| Total Volume : |
27.98 μm3 |
| Max Euclidean Distance : |
38.69 μm |
| Max Path Distance : |
41.27 μm |
| Max Branch Order : |
6 |
| Average Contraction : |
0.96 |
| Total Fragmentation : |
561 |
| Partition Asymmetry : |
0.48 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
62.31° |
| Average Bifurcation Angle Remote : |
55.09° |
| Fractal Dimension : |
1.02 |
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