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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_249732 |
| Cell Name : | PE-8 |
| Archive Name : | Wang_Maas |
| Species Name : | human |
| Strain : | Not applicable |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 30.0 years
|
| Max Age : | 30.0 years
|
| Gender : |
Male
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | Not reported |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | induced pluripotent stem cell-(iPSC)-derived |
| Tertiary Cell Class : | glutamatergic |
| Original Format : | Neurolucida.dat |
| Experiment Protocol : | culture |
| Experimental Condition : | CRISPR/Cas9 edited |
| Staining Method : | immunostaining |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 20x |
| Reconstruction Method : | Neurolucida |
| Date of Deposition : | 2022-10-19 |
| Date of Upload : | 2022-12-06 |
| Persistence Vector : | PE-8.pvec |
| Note : | This reconstruction was obtained from a co-culture of glutamatergic and GABAergic neurons after 49 days in vitro (DIV). CRISPR/Cas9 technology was used to create a heterozygous indel mutation in exon 7 of SETD1A in a healthy hiPSC line derived from a male, 30 years old. Single-cell suspension of hiPSCs was nucleofected with 5 mg of the generated SpCas9-sgRNA plasmid using the P3 Primary Cell 4D-Nucleofector Kit in combination with the 4D Nucleofector Unit X, program CA-137. |
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| Reference Article |
| Related Article Reference : | Loss-of-function variants in the schizophrenia risk gene SETD1A alter neuronal network activity in human neurons through the cAMP/PKA pathway. |
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| Measurements |
| Soma Surface : |
1262.38 μm2 |
| Number of Stems : |
5 |
| Number of Bifurcations : |
24 |
| Number of Branches : |
53 |
| Overall Width : |
323.46 μm |
| Overall Height : |
485.68 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.77 μm |
| Total Length : |
2127.34 μm |
| Total Surface : |
5428.98 μm2 |
| Total Volume : |
2353.65 μm3 |
| Max Euclidean Distance : |
366.06 μm |
| Max Path Distance : |
424.48 μm |
| Max Branch Order : |
8 |
| Average Contraction : |
0.98 |
| Total Fragmentation : |
477 |
| Partition Asymmetry : |
0.58 |
| Average Rall's Ratio : |
1.21 |
| Average Bifurcation Angle Local : |
58.92° |
| Average Bifurcation Angle Remote : |
58.63° |
| Fractal Dimension : |
1.01 |
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0
