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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_149576 |
| Cell Name : | PDGFrB0_5pc |
| Archive Name : | Dolci_Decimo |
| Species Name : | mouse |
| Strain : | CD1 |
| Structural Domains : | Dendrites, No Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 0.0 day
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| Max Age : | 0.0 day
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| Gender : |
Male/Female
|
| Min Weight : | Not reported
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| Max Weight : | Not reported
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| Development : | neonatal |
| Primary Brain Region : | meninges |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | meningeal-derived neural stem cells |
| Tertiary Cell Class : | Platelet-derived growth factor receptor beta (PDGFR-Beta) positive, Microtubule-associated protein 2 (MAP-2) positive |
| Original Format : | Custom.swc |
| Experiment Protocol : | culture |
| Experimental Condition : | alpha-5 treatment |
| Staining Method : | immunostaining |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 100x |
| Reconstruction Method : | Custom |
| Date of Deposition : | 2020-11-03 |
| Date of Upload : | 2021-02-02 |
| Persistence Vector : | PDGFrB0_5pc.pvec |
| Note : | Neuronal cells were supplemented with a mixture (referred to as alpha-5) composed of essential amino acids, TCA intermediates and co-factors (produced by Professional Dietetics, Italy). The mixture was added at 0.5% (w/vol) to achieve final medium concentration as follows: histidine (0.10 mM), isoleucine (0.40 mM), leucine (1.19 mM), lysine (0.58 mM), methionine (30 microM), phenylalanine (60 microM), threonine (0.30 mM), tryptophan (50 microM), valine (0.44 mM), cysteine (0.13 mM), tyrosine (20 microM), thiamine (75 nM), pyridoxine (112 nM) citric acid (0.21 mM), malic acid (70 microM) and succinic acid (80 microM). |
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| Reference Article |
| Related Article Reference : | Complete neural stem cell (NSC) neuronal differentiation requires a branched chain amino acids-induced persistent metabolic shift towards energy metabolism. |
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| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
1 |
| Number of Bifurcations : |
167 |
| Number of Branches : |
335 |
| Overall Width : |
73.85 μm |
| Overall Height : |
95.14 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
963.78 μm |
| Total Surface : |
756.95 μm2 |
| Total Volume : |
47.31 μm3 |
| Max Euclidean Distance : |
95.22 μm |
| Max Path Distance : |
116.11 μm |
| Max Branch Order : |
40 |
| Average Contraction : |
0.91 |
| Total Fragmentation : |
6800 |
| Partition Asymmetry : |
0.79 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
98.67° |
| Average Bifurcation Angle Remote : |
88.01° |
| Fractal Dimension : |
1.06 |
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0
