8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_245803
Cell Name :  P4_CV3_C30
Archive Name :  Guizzetti
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Neurites, Soma
Physical Integrity :  Neurites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  1.0 day
Max Age :  1.0 day
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  neonatal
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Not reported
Original Format :  Neurolucida.dat
Experiment Protocol :  culture
Experimental Condition :  rtPA
Staining Method :  immunostaining
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  40x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-05-05
Date of Upload :  2022-11-07
Persistence Vector :  P4_CV3_C30.pvec
Note :  Three days after sub-culturing astrocytes in the appropriate vessels, medium was changed to DMEM and replaced 24 h later with fresh DMEM/0.1% BSA/penstrep medium containing 50 nM of active recombinant rat tPA for 16h.

Reference Article
Related Article Reference :  Astrocyte tissue plasminogen activator expression during brain development and its role in pyramidal neuron neurite outgrowth.

Measurements
Soma Surface :  402.79 μm2
Number of Stems :  4
Number of Bifurcations :  3
Number of Branches :  10
Overall Width :  77.84 μm
Overall Height :  27.4 μm
Overall Depth :  0.27 μm
Average Diameter :  0.25 μm
Total Length :  151.42 μm
Total Surface :  118.93 μm2
Total Volume :  7.43 μm3
Max Euclidean Distance :  63.38 μm
Max Path Distance :  67.87 μm
Max Branch Order :  2
Average Contraction :  0.92
Total Fragmentation :  37
Partition Asymmetry :  0.33
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  79.73°
Average Bifurcation Angle Remote :  83.39°
Fractal Dimension :  1.02

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