8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_246542
Cell Name :  P2_CV1_27
Archive Name :  Guizzetti
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Neurites, Soma
Physical Integrity :  Neurites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  1.0 day
Max Age :  1.0 day
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  neonatal
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Not reported
Original Format :  Neurolucida.dat
Experiment Protocol :  culture
Experimental Condition :  siPlat
Staining Method :  immunostaining
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  40x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-05-05
Date of Upload :  2022-11-07
Persistence Vector :  P2_CV1_27.pvec
Note :  Two days after sub-culture, astrocytes were transfected in DMEM/0.1% BSA without antibiotics supplemented with 10 microliters/well, containing 1 nM Stealth siRNA targeting the rat Plat. This reconstruction was obtained 48 h after transfection.

Reference Article
Related Article Reference :  Astrocyte tissue plasminogen activator expression during brain development and its role in pyramidal neuron neurite outgrowth.

Measurements
Soma Surface :  536.09 μm2
Number of Stems :  4
Number of Bifurcations :  3
Number of Branches :  10
Overall Width :  105.05 μm
Overall Height :  38.21 μm
Overall Depth :  0.15 μm
Average Diameter :  0.25 μm
Total Length :  260.62 μm
Total Surface :  204.69 μm2
Total Volume :  12.79 μm3
Max Euclidean Distance :  99.86 μm
Max Path Distance :  124.5 μm
Max Branch Order :  2
Average Contraction :  0.92
Total Fragmentation :  62
Partition Asymmetry :  0
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  124.55°
Average Bifurcation Angle Remote :  110.71°
Fractal Dimension :  1.05

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