8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_244131
Cell Name :  P2_CV1_12
Archive Name :  Guizzetti
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Neurites, Soma
Physical Integrity :  Neurites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  1.0 day
Max Age :  1.0 day
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  neonatal
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Not reported
Original Format :  Neurolucida.dat
Experiment Protocol :  culture
Experimental Condition :  siPlat
Staining Method :  immunostaining
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  40x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-05-05
Date of Upload :  2022-11-07
Persistence Vector :  P2_CV1_12.pvec
Note :  Two days after sub-culture, astrocytes were transfected in DMEM/0.1% BSA without antibiotics supplemented with 10 microliters/well, containing 1 nM Stealth siRNA targeting the rat Plat. This reconstruction was obtained 48 h after transfection.

Reference Article
Related Article Reference :  Astrocyte tissue plasminogen activator expression during brain development and its role in pyramidal neuron neurite outgrowth.

Measurements
Soma Surface :  378.88 μm2
Number of Stems :  4
Number of Bifurcations :  6
Number of Branches :  16
Overall Width :  177.83 μm
Overall Height :  51.99 μm
Overall Depth :  0.81 μm
Average Diameter :  0.27 μm
Total Length :  437.61 μm
Total Surface :  371.19 μm2
Total Volume :  25.06 μm3
Max Euclidean Distance :  106.7 μm
Max Path Distance :  112.75 μm
Max Branch Order :  5
Average Contraction :  0.93
Total Fragmentation :  69
Partition Asymmetry :  0.67
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  98.96°
Average Bifurcation Angle Remote :  114.26°
Fractal Dimension :  1.01

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