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Details about selected cell |
NeuroMorpho.Org ID : | NMO_183921 |
Cell Name : | Oct4IR1-1b |
Archive Name : | Medalla |
Species Name : | monkey |
Strain : | Rhesus |
Structural Domains : | Dendrites, Soma, No Axon |
Physical Integrity : | Dendrites Moderate |
Morphological Attributes : | No Diameter, 3D, Angles |
Min Age : | 6.0 years
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Max Age : | 9.0 years
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Gender : |
Male/Female
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Min Weight : | Not reported
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Max Weight : | Not reported
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Development : | adult |
Primary Brain Region : | neocortex |
Secondary Brain Region : | anterior cingulate |
Tertiary Brain Region : | layer 3 |
Primary Cell Class : | principal cell |
Secondary Cell Class : | pyramidal |
Tertiary Cell Class : | dorsal premotor cortex (PMd)-projecting |
Original Format : | Neuronstudio.swc |
Experiment Protocol : | in vitro |
Experimental Condition : | Control |
Staining Method : | Biocytin, Streptavidin-Alexa 405 |
Slicing Direction : | coronal |
Slice Thickness : | 300
μm |
Tissue Shrinkage : |
Not reported
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Objective Type : | oil |
Magnification : | 40x |
Reconstruction Method : | NeuronStudio |
Date of Deposition : | 2022-04-29 |
Date of Upload : | 2022-05-25 |
Persistence Vector : | Oct4IR1-1b.pvec |
Note : | We injected a combination of dextran amine tracers, fluoroemerald and fluororuby. We used a cocktail of the 3KDA and 10kDA isoforms of dextran amines to ensure enhanced bidirectional retrograde and anterograde transport. For PMd injections, the tracer was delivered in 2-3 penetrations (spaced 0.5 mm apart) at a depth of 1.2-1.6 mm below the pial surface of the rostral PMd, to deliver a total of 3-8 microliters per injection site. For each injection, the needle was left in situ for 5-10 mins to allow for diffusion and prevent upward suction of the dye upon retraction of the needle. |
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Reference Article |
Related Article Reference : | Layer-specific pyramidal neuron properties underlie diverse anterior cingulate cortical motor and limbic networks. |
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Measurements |
Soma Surface : |
2237.99 μm2 |
Number of Stems : |
9 |
Number of Bifurcations : |
48 |
Number of Branches : |
105 |
Overall Width : |
205.24 μm |
Overall Height : |
475.13 μm |
Overall Depth : |
70.39 μm |
Average Diameter : |
1.29 μm |
Total Length : |
4901.95 μm |
Total Surface : |
18954.5 μm2 |
Total Volume : |
6599.06 μm3 |
Max Euclidean Distance : |
363.46 μm |
Max Path Distance : |
384.77 μm |
Max Branch Order : |
11 |
Average Contraction : |
0.94 |
Total Fragmentation : |
2343 |
Partition Asymmetry : |
0.43 |
Average Rall's Ratio : |
2.04 |
Average Bifurcation Angle Local : |
68.74° |
Average Bifurcation Angle Remote : |
58.2° |
Fractal Dimension : |
1.02 |
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