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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_183931 |
| Cell Name : | Oct4-IR2b-405C1 |
| Archive Name : | Medalla |
| Species Name : | monkey |
| Strain : | Rhesus |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Moderate |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 6.0 years
|
| Max Age : | 9.0 years
|
| Gender : |
Male/Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | anterior cingulate |
| Tertiary Brain Region : | layer 3 |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | pyramidal |
| Tertiary Cell Class : | dorsal premotor cortex (PMd)-projecting |
| Original Format : | Neuronstudio.swc |
| Experiment Protocol : | in vitro |
| Experimental Condition : | Control |
| Staining Method : | Biocytin, Streptavidin-Alexa 405 |
| Slicing Direction : | coronal |
| Slice Thickness : | 300
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x |
| Reconstruction Method : | NeuronStudio |
| Date of Deposition : | 2022-04-29 |
| Date of Upload : | 2022-05-25 |
| Persistence Vector : | Oct4-IR2b-405C1.pvec |
| Note : | We injected a combination of dextran amine tracers, fluoroemerald and fluororuby. We used a cocktail of the 3KDA and 10kDA isoforms of dextran amines to ensure enhanced bidirectional retrograde and anterograde transport. For PMd injections, the tracer was delivered in 2-3 penetrations (spaced 0.5 mm apart) at a depth of 1.2-1.6 mm below the pial surface of the rostral PMd, to deliver a total of 3-8 microliters per injection site. For each injection, the needle was left in situ for 5-10 mins to allow for diffusion and prevent upward suction of the dye upon retraction of the needle. |
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| Reference Article |
| Related Article Reference : | Layer-specific pyramidal neuron properties underlie diverse anterior cingulate cortical motor and limbic networks. |
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| Measurements |
| Soma Surface : |
1881.13 μm2 |
| Number of Stems : |
12 |
| Number of Bifurcations : |
63 |
| Number of Branches : |
138 |
| Overall Width : |
295.96 μm |
| Overall Height : |
432.01 μm |
| Overall Depth : |
70.1 μm |
| Average Diameter : |
1.06 μm |
| Total Length : |
7347.25 μm |
| Total Surface : |
24625.2 μm2 |
| Total Volume : |
7805.13 μm3 |
| Max Euclidean Distance : |
379.21 μm |
| Max Path Distance : |
424.08 μm |
| Max Branch Order : |
10 |
| Average Contraction : |
0.92 |
| Total Fragmentation : |
4638 |
| Partition Asymmetry : |
0.42 |
| Average Rall's Ratio : |
1.42 |
| Average Bifurcation Angle Local : |
75.46° |
| Average Bifurcation Angle Remote : |
60.62° |
| Fractal Dimension : |
1.02 |
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