8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_178158
Cell Name :  Neo_2M_Light-12
Archive Name :  Masachs
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  2.0 months
Max Age :  2.0 months
Gender :  Male
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  hippocampus
Secondary Brain Region :  dentate gyrus
Tertiary Brain Region :  granule layer
Primary Cell Class :  principal cell
Secondary Cell Class :  granule
Tertiary Cell Class :  born at PND3
Original Format :  Neurolucida.dat
Experiment Protocol :  in vivo
Experimental Condition :  Optogenetic stimulation
Staining Method :  enhanced green fluorescent protein, immunostaining
Slicing Direction :  coronal
Slice Thickness :  50  μm
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  100x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-02-16
Date of Upload :  2022-02-23
Persistence Vector :  Neo_2M_Light-12.pvec
Note :  The retrovirus expressing an inhibitory optogene Archaerhodopsin-3 (Arch-EGFP) was injected bilaterally into the dorsal area of the septal DG at postnatal day 3. For Neo-DGNs groups, [AP]: -1.2 mm; lateral [L]: +/-1.35 mm; [P]: -2.6 mm. 1 microliter of viral suspension were injected at a flow rate 0.25 L/min. Illumination was applied throughout the session at 1 Hz pulses of 750 ms. This reconstruction was obtained 2 months after BrdU injection.

Reference Article
Related Article Reference :  The temporal origin of dentate granule neurons dictates their role in spatial memory.

Measurements
Soma Surface :  578.56 μm2
Number of Stems :  1
Number of Bifurcations :  7
Number of Branches :  15
Overall Width :  92.64 μm
Overall Height :  238.85 μm
Overall Depth :  28.03 μm
Average Diameter :  0.07 μm
Total Length :  661.55 μm
Total Surface :  145.48 μm2
Total Volume :  2.55 μm3
Max Euclidean Distance :  255.08 μm
Max Path Distance :  276.01 μm
Max Branch Order :  5
Average Contraction :  0.89
Total Fragmentation :  191
Partition Asymmetry :  0.45
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  53.12°
Average Bifurcation Angle Remote :  46.16°
Fractal Dimension :  1.03

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