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Details about selected cell |
NeuroMorpho.Org ID : | NMO_151324 |
Cell Name : | NRas-KD-NRas-WT1 |
Archive Name : | Shimell |
Species Name : | rat |
Strain : | Sprague-Dawley |
Structural Domains : | Dendrites, No Soma, No Axon |
Physical Integrity : | Dendrites Moderate |
Morphological Attributes : | No Diameter, 2D, Angles |
Min Age : | 18.0 days
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Max Age : | 18.0 days
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Gender : |
Male/Female
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Min Weight : | Not reported
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Max Weight : | Not reported
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Development : | embryonic |
Primary Brain Region : | hippocampus |
Secondary Brain Region : | Not reported |
Tertiary Brain Region : | Not reported |
Primary Cell Class : | principal cell |
Secondary Cell Class : | pyramidal |
Tertiary Cell Class : | Not reported |
Original Format : | NeuronJ.ndf |
Experiment Protocol : | culture |
Experimental Condition : | N-Ras shRNA + N-Ras WT |
Staining Method : | enhanced green fluorescent protein |
Slicing Direction : | Not applicable |
Slice Thickness : | Not applicable
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Tissue Shrinkage : |
Not reported
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Objective Type : | oil |
Magnification : | 20x |
Reconstruction Method : | NeuronJ |
Date of Deposition : | 2020-04-20 |
Date of Upload : | 2021-02-25 |
Persistence Vector : | NRas-KD-NRas-WT1.pvec |
Note : | Hippocampal neurons were transfected at 10DIV with eGFP and Zdhhc9 shRNA plus the palmitoylation-deficient N-Ras (1-3 mg of total plasmid DNA was added) and taken for analysis at 13-14 DIV. |
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Reference Article |
Related Article Reference : | The X-Linked Intellectual Disability Gene Zdhhc9 Is Essential for Dendrite Outgrowth and Inhibitory Synapse Formation. |
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Measurements |
Soma Surface : |
N/A |
Number of Stems : |
2 |
Number of Bifurcations : |
13 |
Number of Branches : |
28 |
Overall Width : |
159.83 μm |
Overall Height : |
351.23 μm |
Overall Depth : |
0 μm |
Average Diameter : |
0.25 μm |
Total Length : |
1304.4 μm |
Total Surface : |
1024.47 μm2 |
Total Volume : |
64.03 μm3 |
Max Euclidean Distance : |
276.38 μm |
Max Path Distance : |
332.93 μm |
Max Branch Order : |
8 |
Average Contraction : |
0.95 |
Total Fragmentation : |
377 |
Partition Asymmetry : |
0.47 |
Average Rall's Ratio : |
2 |
Average Bifurcation Angle Local : |
92.25° |
Average Bifurcation Angle Remote : |
96.87° |
Fractal Dimension : |
1.01 |
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