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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_284371 |
| Cell Name : | MsJinx13_s2_40HzFlicker_1h_IBA1_NFkBinh_04 |
| Archive Name : | Prichard_Singer |
| Species Name : | mouse |
| Strain : | C57BL/6J |
| Structural Domains : | Processes, Soma |
| Physical Integrity : | Processes Complete |
| Morphological Attributes : | Diameter, 3D, Angles |
| Min Age : | 2.0 months
|
| Max Age : | 2.0 months
|
| Gender : |
Male
|
| Min Weight : | 30 grams
|
| Max Weight : | 30 grams
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | occipital |
| Tertiary Brain Region : | primary visual, right |
| Primary Cell Class : | Glia |
| Secondary Cell Class : | microglia |
| Tertiary Cell Class : | Iba1-positive |
| Original Format : | Imaris.ims |
| Experiment Protocol : | in vitro |
| Experimental Condition : | visual flicker + NF-kB inhibitor |
| Staining Method : | immunostaining |
| Slicing Direction : | Sagittal |
| Slice Thickness : | 40
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 20x , 40x |
| Reconstruction Method : | Imaris |
| Date of Deposition : | 2023-07-28 |
| Date of Upload : | 2024-07-30 |
| Persistence Vector : | MsJinx13_s2_40HzFlicker_1h_IBA1_NFkBinh_04.pvec |
| Note : | Animals were exposed to LED lights flashing at 40 Hz frequency (50% duty cycle: 12.5-ms light on and 12.5-ms light off), for 1 hour. The NF-kB pathway was inhibited using inhibitor of NF-kB kinase inhibitor (IMD0354 at 10 mg/kg) combined with curcumin (S1848 at 100 mg/kg). All drugs were solubilized in vehicle consisting of dimethyl sulfoxide, polyethylene glycol, polysorbate 20 (Tween 20), and sterile physiologic saline. Drugs were delivered via intraperitoneal injection after the start of 1 hour of flicker stimulation. |
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| Reference Article |
| Related Article Reference : | Brain rhythms control microglial response and cytokine expression via NF-κB signaling. |
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| Measurements |
| Soma Surface : |
224.12 μm2 |
| Number of Stems : |
11 |
| Number of Bifurcations : |
27 |
| Number of Branches : |
65 |
| Overall Width : |
10.63 μm |
| Overall Height : |
34.83 μm |
| Overall Depth : |
38.84 μm |
| Average Diameter : |
1.16 μm |
| Total Length : |
296.02 μm |
| Total Surface : |
1084.49 μm2 |
| Total Volume : |
385.87 μm3 |
| Max Euclidean Distance : |
32.91 μm |
| Max Path Distance : |
45.19 μm |
| Max Branch Order : |
7 |
| Average Contraction : |
0.89 |
| Total Fragmentation : |
1181 |
| Partition Asymmetry : |
0.57 |
| Average Rall's Ratio : |
2.1 |
| Average Bifurcation Angle Local : |
50.09° |
| Average Bifurcation Angle Remote : |
60.48° |
| Fractal Dimension : |
1.06 |
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