8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_283889
Cell Name :  Ms259_20HzFlicker_1h_IBA1_veh_10
Archive Name :  Prichard_Singer
Species Name :  mouse
Strain :  C57BL/6J
Structural Domains :  Processes, Soma
Physical Integrity :  Processes Complete
Morphological Attributes :  Diameter, 3D, Angles
Min Age :  2.0 months
Max Age :  2.0 months
Gender :  Male
Min Weight :  30 grams
Max Weight :  30 grams
Development :  adult
Primary Brain Region :  neocortex
Secondary Brain Region :  occipital
Tertiary Brain Region :  primary visual, right
Primary Cell Class :  Glia
Secondary Cell Class :  microglia
Tertiary Cell Class :  Iba1-positive
Original Format :  Imaris.ims
Experiment Protocol :  in vitro
Experimental Condition :  visual flicker
Staining Method :  immunostaining
Slicing Direction :  Sagittal
Slice Thickness :  40  μm
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  20x , 40x
Reconstruction Method :  Imaris
Date of Deposition :  2023-07-28
Date of Upload :  2024-07-30
Persistence Vector :  Ms259_20HzFlicker_1h_IBA1_veh_10.pvec
Note :  Vehicle was delivered via intraperitoneal injection 30 min before the start of 1 hour of flicker stimulation, consisting of dimethyl sulfoxide, polyethylene glycol, polysorbate 20 (Tween 20), and sterile physiologic saline. Animals were exposed to LED lights flashing at 20 Hz frequency (50% duty cycle: 25-ms light on and 25-ms light off), for 1 hour.

Reference Article
Related Article Reference :  Brain rhythms control microglial response and cytokine expression via NF-κB signaling.

Measurements
Soma Surface :  250.51 μm2
Number of Stems :  7
Number of Bifurcations :  16
Number of Branches :  39
Overall Width :  15.44 μm
Overall Height :  31.88 μm
Overall Depth :  11.9 μm
Average Diameter :  1.18 μm
Total Length :  197.47 μm
Total Surface :  773.16 μm2
Total Volume :  299.84 μm3
Max Euclidean Distance :  25.46 μm
Max Path Distance :  31.65 μm
Max Branch Order :  6
Average Contraction :  0.89
Total Fragmentation :  915
Partition Asymmetry :  0.51
Average Rall's Ratio :  2.01
Average Bifurcation Angle Local :  51.86°
Average Bifurcation Angle Remote :  65.4°
Fractal Dimension :  1.05

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