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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_284777 |
| Cell Name : | Ms253_40HzFlicker_1h_IBA1_NFkBinh_09 |
| Archive Name : | Prichard_Singer |
| Species Name : | mouse |
| Strain : | C57BL/6J |
| Structural Domains : | Processes, Soma |
| Physical Integrity : | Processes Complete |
| Morphological Attributes : | Diameter, 3D, Angles |
| Min Age : | 2.0 months
|
| Max Age : | 2.0 months
|
| Gender : |
Male
|
| Min Weight : | 30 grams
|
| Max Weight : | 30 grams
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | occipital |
| Tertiary Brain Region : | primary visual, right |
| Primary Cell Class : | Glia |
| Secondary Cell Class : | microglia |
| Tertiary Cell Class : | Iba1-positive |
| Original Format : | Imaris.ims |
| Experiment Protocol : | in vitro |
| Experimental Condition : | visual flicker + NF-kB inhibitor |
| Staining Method : | immunostaining |
| Slicing Direction : | Sagittal |
| Slice Thickness : | 40
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 20x , 40x |
| Reconstruction Method : | Imaris |
| Date of Deposition : | 2023-07-28 |
| Date of Upload : | 2024-07-30 |
| Persistence Vector : | Ms253_40HzFlicker_1h_IBA1_NFkBinh_09.pvec |
| Note : | Animals were exposed to LED lights flashing at 40 Hz frequency (50% duty cycle: 12.5-ms light on and 12.5-ms light off), for 1 hour. The NF-kB pathway was inhibited using inhibitor of NF-kB kinase inhibitor (IMD0354 at 10 mg/kg) combined with curcumin (S1848 at 100 mg/kg). All drugs were solubilized in vehicle consisting of dimethyl sulfoxide, polyethylene glycol, polysorbate 20 (Tween 20), and sterile physiologic saline. Drugs were delivered via intraperitoneal injection 30 minutes before the start of 1 hour of flicker stimulation. |
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| Reference Article |
| Related Article Reference : | Brain rhythms control microglial response and cytokine expression via NF-κB signaling. |
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| Measurements |
| Soma Surface : |
528.3 μm2 |
| Number of Stems : |
16 |
| Number of Bifurcations : |
59 |
| Number of Branches : |
134 |
| Overall Width : |
13.23 μm |
| Overall Height : |
44.2 μm |
| Overall Depth : |
34.38 μm |
| Average Diameter : |
1.2 μm |
| Total Length : |
524.83 μm |
| Total Surface : |
2011.93 μm2 |
| Total Volume : |
781.46 μm3 |
| Max Euclidean Distance : |
30.88 μm |
| Max Path Distance : |
45.87 μm |
| Max Branch Order : |
13 |
| Average Contraction : |
0.89 |
| Total Fragmentation : |
2225 |
| Partition Asymmetry : |
0.63 |
| Average Rall's Ratio : |
2.01 |
| Average Bifurcation Angle Local : |
64.01° |
| Average Bifurcation Angle Remote : |
69.96° |
| Fractal Dimension : |
1.06 |
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