8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_284509
Cell Name :  Ms251_40HzFlicker_1h_IBA1_veh_04
Archive Name :  Prichard_Singer
Species Name :  mouse
Strain :  C57BL/6J
Structural Domains :  Processes, Soma
Physical Integrity :  Processes Complete
Morphological Attributes :  Diameter, 3D, Angles
Min Age :  2.0 months
Max Age :  2.0 months
Gender :  Male
Min Weight :  30 grams
Max Weight :  30 grams
Development :  adult
Primary Brain Region :  neocortex
Secondary Brain Region :  occipital
Tertiary Brain Region :  primary visual, right
Primary Cell Class :  Glia
Secondary Cell Class :  microglia
Tertiary Cell Class :  Iba1-positive
Original Format :  Imaris.ims
Experiment Protocol :  in vitro
Experimental Condition :  visual flicker
Staining Method :  immunostaining
Slicing Direction :  Sagittal
Slice Thickness :  40  μm
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  20x , 40x
Reconstruction Method :  Imaris
Date of Deposition :  2023-07-28
Date of Upload :  2024-07-30
Persistence Vector :  Ms251_40HzFlicker_1h_IBA1_veh_04.pvec
Note :  Animals were exposed to LED lights flashing at 40 Hz frequency (50% duty cycle: 12.5-ms light on and 12.5-ms light off), for 1 hour. Vehicle was delivered via intraperitoneal injection 30 min before the start of 1 hour of flicker stimulation, consisting of dimethyl sulfoxide, polyethylene glycol, polysorbate 20 (Tween 20), and sterile physiologic saline.

Reference Article
Related Article Reference :  Brain rhythms control microglial response and cytokine expression via NF-κB signaling.

Measurements
Soma Surface :  519.6 μm2
Number of Stems :  13
Number of Bifurcations :  23
Number of Branches :  59
Overall Width :  15.37 μm
Overall Height :  43.76 μm
Overall Depth :  26.44 μm
Average Diameter :  1.05 μm
Total Length :  283.89 μm
Total Surface :  958.31 μm2
Total Volume :  303.4 μm3
Max Euclidean Distance :  27.92 μm
Max Path Distance :  35.37 μm
Max Branch Order :  5
Average Contraction :  0.88
Total Fragmentation :  1489
Partition Asymmetry :  0.47
Average Rall's Ratio :  1.97
Average Bifurcation Angle Local :  71.59°
Average Bifurcation Angle Remote :  82.33°
Fractal Dimension :  1.05

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