|
Details about selected cell |
NeuroMorpho.Org ID : | NMO_183937 |
Cell Name : | May22-IR4a-40x-lowCorrectScale |
Archive Name : | Medalla |
Species Name : | monkey |
Strain : | Rhesus |
Structural Domains : | Dendrites, Soma, No Axon |
Physical Integrity : | Dendrites Moderate |
Morphological Attributes : | No Diameter, 3D, Angles |
Min Age : | 6.0 years
|
Max Age : | 9.0 years
|
Gender : |
Male/Female
|
Min Weight : | Not reported
|
Max Weight : | Not reported
|
Development : | adult |
Primary Brain Region : | neocortex |
Secondary Brain Region : | anterior cingulate |
Tertiary Brain Region : | layer 3 |
Primary Cell Class : | principal cell |
Secondary Cell Class : | pyramidal |
Tertiary Cell Class : | amygdala-projecting |
Original Format : | Neurolucida.dat |
Experiment Protocol : | in vitro |
Experimental Condition : | Control |
Staining Method : | Biocytin, Streptavidin-Alexa 405 |
Slicing Direction : | coronal |
Slice Thickness : | 300
μm |
Tissue Shrinkage : |
Not reported
|
Objective Type : | oil |
Magnification : | 40x |
Reconstruction Method : | Neurolucida |
Date of Deposition : | 2022-04-29 |
Date of Upload : | 2022-05-25 |
Persistence Vector : | May22-IR4a-40x-lowCorrectScale.pvec |
Note : | We injected a combination of dextran amine tracers, fluoroemerald and fluororuby. We used a cocktail of the 3KDA and 10kDA isoforms of dextran amines to ensure enhanced bidirectional retrograde and anterograde transport. For AMY injections, one penetration was made, delivering a total of 3 microliters of tracer into the basolateral complex. For each injection, the needle was left in situ for 5-10 mins to allow for diffusion and prevent upward suction of the dye upon retraction of the needle. |
|
|
Reference Article |
Related Article Reference : | Layer-specific pyramidal neuron properties underlie diverse anterior cingulate cortical motor and limbic networks. |
|
|
|
Measurements |
Soma Surface : |
817.78 μm2 |
Number of Stems : |
9 |
Number of Bifurcations : |
64 |
Number of Branches : |
137 |
Overall Width : |
251.52 μm |
Overall Height : |
391.34 μm |
Overall Depth : |
75.24 μm |
Average Diameter : |
0.98 μm |
Total Length : |
9376.49 μm |
Total Surface : |
29128.2 μm2 |
Total Volume : |
7657.37 μm3 |
Max Euclidean Distance : |
284.28 μm |
Max Path Distance : |
332.28 μm |
Max Branch Order : |
12 |
Average Contraction : |
0.86 |
Total Fragmentation : |
16072 |
Partition Asymmetry : |
0.42 |
Average Rall's Ratio : |
1.87 |
Average Bifurcation Angle Local : |
69.52° |
Average Bifurcation Angle Remote : |
63.51° |
Fractal Dimension : |
1.04 |
|
|
|
|
|