|
| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_173115 |
| Cell Name : | MAX_7-KOTTR-GFP_3-MsTTR55_A3_2_1 |
| Archive Name : | Gomes |
| Species Name : | mouse |
| Strain : | TTR -/- |
| Structural Domains : | Neurites, Soma |
| Physical Integrity : | Neurites Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 16.0 days
|
| Max Age : | 16.0 days
|
| Gender : |
Male/Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | Not reported |
| Tertiary Cell Class : | Not reported |
| Original Format : | NeuronJ.ndf |
| Experiment Protocol : | culture |
| Experimental Condition : | megalin overexpression + transthyretin (TTR) stimulation |
| Staining Method : | green fluorescent protein |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x |
| Reconstruction Method : | NeuronJ |
| Date of Deposition : | 2021-10-07 |
| Date of Upload : | 2022-01-12 |
| Persistence Vector : | MAX_7-KOTTR-GFP_3-MsTTR55_A3_2_1.pvec |
| Note : | TTR KO cultured hippocampal neurons (11 DIV) were co-transfected with GFP (pEGFP) and mini-megalin plasmid (pLNCX-M4) for 48 h, and stimulated with recombinant mouse TTR (55 mg/ml) for 24 h. |
|
|
| Reference Article |
| Related Article Reference : | Neuronal megalin mediates synaptic plasticity-a novel mechanism underlying intellectual disabilities in megalin gene pathologies. |
|
|
|
|
| Measurements |
| Soma Surface : |
2250.77 μm2 |
| Number of Stems : |
17 |
| Number of Bifurcations : |
15 |
| Number of Branches : |
47 |
| Overall Width : |
169.83 μm |
| Overall Height : |
127.08 μm |
| Overall Depth : |
1.35 μm |
| Average Diameter : |
1 μm |
| Total Length : |
1602.28 μm |
| Total Surface : |
5033.71 μm2 |
| Total Volume : |
1258.43 μm3 |
| Max Euclidean Distance : |
158.8 μm |
| Max Path Distance : |
218.38 μm |
| Max Branch Order : |
3 |
| Average Contraction : |
0.94 |
| Total Fragmentation : |
291 |
| Partition Asymmetry : |
0.33 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
35.17° |
| Average Bifurcation Angle Remote : |
43° |
| Fractal Dimension : |
1.02 |
|
|
|
|
|
0
