8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_161890
Cell Name :  Live4-2-2019_12-28-47_PM_denoised
Archive Name :  Ruthazer
Species Name :  Xenopus laevis
Strain :  Albino
Structural Domains :  Dendrites, No Soma, No Axon
Physical Integrity :  Dendrites Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  42.0 days
Max Age :  44.0 days
Gender :  Not reported
Min Weight :  Not reported
Max Weight :  Not reported
Development :  tadpole
Primary Brain Region :  mesencephalon
Secondary Brain Region :  tectum
Tertiary Brain Region :  optic
Primary Cell Class :  principal cell
Secondary Cell Class :  Not reported
Tertiary Cell Class :  Not reported
Original Format :  Imaris.imx
Experiment Protocol :  in vivo
Experimental Condition :  Control
Staining Method :  enhanced green fluorescent protein
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  water
Magnification :  60x
Reconstruction Method :  Imaris
Date of Deposition :  2021-01-20
Date of Upload :  2021-08-06
Persistence Vector :  Live4-2-2019_12-28-47_PM_denoised.pvec
Note :  Albino tadpoles at stage 44-46 according to the criteria of Nieuwkoop and Faber (1956) were used for electroporation. Animals were anesthetized by immersion in MS222 (0.02% in 0.1 x MBS-H). DNA solution containing various concentrations of either pEGFP-N1 or a mixture of pCAG-Cre with 1 micrograms/microliter. pCALNL-GFP and/or pCALNL-DsRed suspended in distilled water with a small amount of fast green dye for visualization was pressure injected in the brain ventricle with a glass micropipette. Reconstructions were obtained 5 days after the cre Mediated single-cell electroporation

Reference Article
Related Article Reference :  A Simple and Efficient Method for Visualizing Individual Cells in vivo by Cre-Mediated Single-Cell Labeling by Electroporation (CREMSCLE).

Measurements
Soma Surface :  N/A
Number of Stems :  1
Number of Bifurcations :  54
Number of Branches :  109
Overall Width :  52.73 μm
Overall Height :  76.54 μm
Overall Depth :  8.21 μm
Average Diameter :  0.81 μm
Total Length :  645.99 μm
Total Surface :  1634.94 μm2
Total Volume :  329.28 μm3
Max Euclidean Distance :  89.75 μm
Max Path Distance :  98 μm
Max Branch Order :  14
Average Contraction :  0.95
Total Fragmentation :  2260
Partition Asymmetry :  0.68
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  45.27°
Average Bifurcation Angle Remote :  70.9°
Fractal Dimension :  1.02

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