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Details about selected cell |
NeuroMorpho.Org ID : | NMO_161889 |
Cell Name : | Live12-6-2018_2-05-43_PM_denoised |
Archive Name : | Ruthazer |
Species Name : | Xenopus laevis |
Strain : | Albino |
Structural Domains : | Dendrites, No Soma, No Axon |
Physical Integrity : | Dendrites Moderate |
Morphological Attributes : | No Diameter, 3D, Angles |
Min Age : | 42.0 days
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Max Age : | 44.0 days
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Gender : |
Not reported
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Min Weight : | Not reported
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Max Weight : | Not reported
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Development : | tadpole |
Primary Brain Region : | mesencephalon |
Secondary Brain Region : | tectum |
Tertiary Brain Region : | optic |
Primary Cell Class : | principal cell |
Secondary Cell Class : | Not reported |
Tertiary Cell Class : | Not reported |
Original Format : | Imaris.imx |
Experiment Protocol : | in vivo |
Experimental Condition : | Control |
Staining Method : | enhanced green fluorescent protein |
Slicing Direction : | Not applicable |
Slice Thickness : | Not applicable
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Tissue Shrinkage : |
Not reported
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Objective Type : | water |
Magnification : | 60x |
Reconstruction Method : | Imaris |
Date of Deposition : | 2021-01-20 |
Date of Upload : | 2021-08-06 |
Persistence Vector : | Live12-6-2018_2-05-43_PM_denoised.pvec |
Note : | Albino tadpoles at stage 44-46 according to the criteria of Nieuwkoop and Faber (1956) were used for electroporation. Animals were anesthetized by immersion in MS222 (0.02% in 0.1 x MBS-H). DNA solution containing various concentrations of either pEGFP-N1 or a mixture of pCAG-Cre with 1 micrograms/microliter. pCALNL-GFP and/or pCALNL-DsRed suspended in distilled water with a small amount of fast green dye for visualization was pressure injected in the brain ventricle with a glass micropipette. Reconstructions were obtained 5 days after the cre Mediated single-cell electroporation |
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Reference Article |
Related Article Reference : | A Simple and Efficient Method for Visualizing Individual Cells in vivo by Cre-Mediated Single-Cell Labeling by Electroporation (CREMSCLE). |
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Measurements |
Soma Surface : |
N/A |
Number of Stems : |
2 |
Number of Bifurcations : |
51 |
Number of Branches : |
104 |
Overall Width : |
43.77 μm |
Overall Height : |
94.47 μm |
Overall Depth : |
5.22 μm |
Average Diameter : |
0.81 μm |
Total Length : |
599.87 μm |
Total Surface : |
1518.21 μm2 |
Total Volume : |
305.77 μm3 |
Max Euclidean Distance : |
110.62 μm |
Max Path Distance : |
178.23 μm |
Max Branch Order : |
20 |
Average Contraction : |
0.93 |
Total Fragmentation : |
2159 |
Partition Asymmetry : |
0.77 |
Average Rall's Ratio : |
2 |
Average Bifurcation Angle Local : |
51.89° |
Average Bifurcation Angle Remote : |
85.71° |
Fractal Dimension : |
1.03 |
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