8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_161891
Cell Name :  Live1-19-2019_7-02-28_PM_denoised
Archive Name :  Ruthazer
Species Name :  Xenopus laevis
Strain :  Albino
Structural Domains :  Dendrites, No Soma, No Axon
Physical Integrity :  Dendrites Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  42.0 days
Max Age :  44.0 days
Gender :  Not reported
Min Weight :  Not reported
Max Weight :  Not reported
Development :  tadpole
Primary Brain Region :  mesencephalon
Secondary Brain Region :  tectum
Tertiary Brain Region :  optic
Primary Cell Class :  principal cell
Secondary Cell Class :  Not reported
Tertiary Cell Class :  Not reported
Original Format :  Imaris.imx
Experiment Protocol :  in vivo
Experimental Condition :  Control
Staining Method :  enhanced green fluorescent protein
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  water
Magnification :  60x
Reconstruction Method :  Imaris
Date of Deposition :  2021-01-20
Date of Upload :  2021-08-06
Persistence Vector :  Live1-19-2019_7-02-28_PM_denoised.pvec
Note :  Albino tadpoles at stage 44-46 according to the criteria of Nieuwkoop and Faber (1956) were used for electroporation. Animals were anesthetized by immersion in MS222 (0.02% in 0.1 x MBS-H). DNA solution containing various concentrations of either pEGFP-N1 or a mixture of pCAG-Cre with 1 micrograms/microliter. pCALNL-GFP and/or pCALNL-DsRed suspended in distilled water with a small amount of fast green dye for visualization was pressure injected in the brain ventricle with a glass micropipette. Reconstructions were obtained 5 days after the cre Mediated single-cell electroporation

Reference Article
Related Article Reference :  A Simple and Efficient Method for Visualizing Individual Cells in vivo by Cre-Mediated Single-Cell Labeling by Electroporation (CREMSCLE).

Measurements
Soma Surface :  N/A
Number of Stems :  1
Number of Bifurcations :  38
Number of Branches :  77
Overall Width :  56.09 μm
Overall Height :  58.07 μm
Overall Depth :  10.35 μm
Average Diameter :  0.81 μm
Total Length :  518.45 μm
Total Surface :  1312.14 μm2
Total Volume :  264.27 μm3
Max Euclidean Distance :  82.97 μm
Max Path Distance :  102.88 μm
Max Branch Order :  14
Average Contraction :  0.93
Total Fragmentation :  1769
Partition Asymmetry :  0.51
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  49.66°
Average Bifurcation Angle Remote :  81.83°
Fractal Dimension :  1.03

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