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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_158545 |
| Cell Name : | KingLui_XII-1 |
| Archive Name : | Schornig_Taverna |
| Species Name : | Bonobo |
| Strain : | Not reported |
| Structural Domains : | Neurites, No Soma |
| Physical Integrity : | Neurites Complete |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 26.0 days
|
| Max Age : | 26.0 days
|
| Gender : |
Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | not reported |
| Primary Brain Region : | peripheral nervous system |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | induced pluripotent stem cell-(iPSC)-derived |
| Tertiary Cell Class : | Not reported |
| Original Format : | Imaris.hoc |
| Experiment Protocol : | culture |
| Experimental Condition : | Control |
| Staining Method : | green fluorescent protein |
| Slicing Direction : | Not reported |
| Slice Thickness : | None
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x , 63x |
| Reconstruction Method : | Imaris |
| Date of Deposition : | 2021-05-19 |
| Date of Upload : | 2021-07-02 |
| Persistence Vector : | KingLui_XII-1.pvec |
| Note : | The bonobo iPSCs line BmRNA was generated in a previous study (Kanton et al., Nature, 2019). To generate GFP-expressing iNs, cells were lipofected 4 days before fixation using the Lipofectamine 3000 Transfection Kit. Briefly, 5 microliters OptiMEM was mixed with 0.75 microliters lipofectamine and then added to 25 microliters OptiMEM containing 0.1 microgram GFP pMAX plasmid (Lonza) and 1 microliter P3000 reagent. After incubation for 15 min, 50 microliters lipofection mix was added per coverslip, and the iNs were kept in culture until fixation. GFP-pmax-lipofected iNs were fixed at day 14 of differentiation. This reconstruction is from culture 14 days after GFP-Transfection. iNeurons are described as a mixture of upper layer cortical neurons (positive for CUX1; BRN2), sensory neurons (postive for PRPH, ISL1) and sensory neurons of the cortex (postive for both cortical and sensory neurons). |
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| Reference Article |
| Related Article Reference : | Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes. |
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| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
2 |
| Number of Bifurcations : |
12 |
| Number of Branches : |
26 |
| Overall Width : |
5318.32 μm |
| Overall Height : |
4289.13 μm |
| Overall Depth : |
3.15 μm |
| Average Diameter : |
10.24 μm |
| Total Length : |
23489.6 μm |
| Total Surface : |
755638 μm2 |
| Total Volume : |
1938520 μm3 |
| Max Euclidean Distance : |
5729.09 μm |
| Max Path Distance : |
9917.21 μm |
| Max Branch Order : |
9 |
| Average Contraction : |
0.89 |
| Total Fragmentation : |
1502 |
| Partition Asymmetry : |
0.6 |
| Average Rall's Ratio : |
1.99 |
| Average Bifurcation Angle Local : |
43.05° |
| Average Bifurcation Angle Remote : |
80.42° |
| Fractal Dimension : |
1.01 |
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