8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_177453
Cell Name :  H286_neuron2_reg
Archive Name :  Guo
Species Name :  mouse
Strain :  C57BL/6J
Structural Domains :  Dendrites, Soma, Axon
Physical Integrity :  Dendrites & Axon Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  2.0 months
Max Age :  2.0 months
Gender :  Male
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  neocortex
Secondary Brain Region :  medial prefrontal
Tertiary Brain Region :  prelimbic, layer 5
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Extratelencephalic (ET)
Original Format :  Vaa3D.swc
Experiment Protocol :  in vivo
Experimental Condition :  Control
Staining Method :  AAV + eGFP
Slicing Direction :  transverse
Slice Thickness :  1000  μm
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  Multiple
Reconstruction Method :  Vaa3D
Date of Deposition :  2022-01-06
Date of Upload :  2022-02-11
Persistence Vector :  H286_neuron2_reg.pvec
Note :  To achieve sparse labeling of a few dozens of cortical neurons, 50 nl of AAV2/1-hSyn-Cre and AAV2/1-CAG-Flex-EGFP was injected into mPFC (AP: +1.85, ML: 0.4, DV: 1.7) unilaterally at a rate of 17 nl/min. Mice were maintained for 5 weeks to allow a stable and strong expression of EGFP.

Reference Article
Related Article Reference :  Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging.

Measurements
Soma Surface :  377.01 μm2
Number of Stems :  9
Number of Bifurcations :  138
Number of Branches :  285
Overall Width :  4173.03 μm
Overall Height :  5571.04 μm
Overall Depth :  2711.64 μm
Average Diameter :  2 μm
Total Length :  81484.7 μm
Total Surface :  511991 μm2
Total Volume :  256000 μm3
Max Euclidean Distance :  7158.56 μm
Max Path Distance :  10396.4 μm
Max Branch Order :  23
Average Contraction :  0.87
Total Fragmentation :  8329
Partition Asymmetry :  0.59
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  81.39°
Average Bifurcation Angle Remote :  75.97°
Fractal Dimension :  1.02

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