8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_177432
Cell Name :  H285_neuron2_reg
Archive Name :  Guo
Species Name :  mouse
Strain :  C57BL/6J
Structural Domains :  Dendrites, Soma, Axon
Physical Integrity :  Dendrites & Axon Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  2.0 months
Max Age :  2.0 months
Gender :  Male
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  neocortex
Secondary Brain Region :  medial prefrontal
Tertiary Brain Region :  prelimbic, layer 2-3
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Intratelencephalic (IT)
Original Format :  Vaa3D.swc
Experiment Protocol :  in vivo
Experimental Condition :  Control
Staining Method :  AAV + eGFP
Slicing Direction :  transverse
Slice Thickness :  1000  μm
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  Multiple
Reconstruction Method :  Vaa3D
Date of Deposition :  2022-01-06
Date of Upload :  2022-02-11
Persistence Vector :  H285_neuron2_reg.pvec
Note :  To achieve sparse labeling of a few dozens of cortical neurons, 50 nl of AAV2/1-hSyn-Cre and AAV2/1-CAG-Flex-EGFP was injected into mPFC (AP: +1.85, ML: 0.4, DV: 1.7) unilaterally at a rate of 17 nl/min. Mice were maintained for 5 weeks to allow a stable and strong expression of EGFP.

Reference Article
Related Article Reference :  Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging.

Measurements
Soma Surface :  783.84 μm2
Number of Stems :  11
Number of Bifurcations :  165
Number of Branches :  341
Overall Width :  6650.57 μm
Overall Height :  4995.3 μm
Overall Depth :  3192.58 μm
Average Diameter :  2 μm
Total Length :  110839 μm
Total Surface :  696425 μm2
Total Volume :  348213 μm3
Max Euclidean Distance :  4896.1 μm
Max Path Distance :  10136.7 μm
Max Branch Order :  18
Average Contraction :  0.87
Total Fragmentation :  6767
Partition Asymmetry :  0.5
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  76.78°
Average Bifurcation Angle Remote :  70.3°
Fractal Dimension :  1.03

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