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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_177460 |
| Cell Name : | H244_neuron2_reg |
| Archive Name : | Guo |
| Species Name : | mouse |
| Strain : | C57BL/6J |
| Structural Domains : | Dendrites, Soma, Axon |
| Physical Integrity : | Dendrites & Axon Moderate |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 2.0 months
|
| Max Age : | 2.0 months
|
| Gender : |
Male
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | medial prefrontal |
| Tertiary Brain Region : | prelimbic, layer 5 |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | pyramidal |
| Tertiary Cell Class : | pyramidal tract (PT) cell Sim1+ |
| Original Format : | Vaa3D.swc |
| Experiment Protocol : | in vivo |
| Experimental Condition : | Control |
| Staining Method : | AAV + eGFP |
| Slicing Direction : | horizontal |
| Slice Thickness : | 1
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x |
| Reconstruction Method : | Vaa3D |
| Date of Deposition : | 2021-11-16 |
| Date of Upload : | 2022-02-11 |
| Persistence Vector : | H244_neuron2_reg.pvec |
| Note : | To achieve sparse labeling of a few dozens of cortical neurons, a mixture of AAV2/1-hSyn-Cre and AAV2/1-CAG-Flex-EGFP was injected. The viral mixture was delivered through an oil hydraulic micromanipulator (Narishige, MO-10, 17 nL/min for 50 nL) into mPFC (injection site, AP: +1.85, ML: 0.4, DV: 1.7). Each mouse was injected unilaterally and maintained for 5 weeks to allow stable and strong expression of EGFP. |
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| Reference Article |
| Related Article Reference : | Sparse imaging and reconstruction tomography for high-speed high-resolution whole-brain imaging |
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| Measurements |
| Soma Surface : |
411.87 μm2 |
| Number of Stems : |
10 |
| Number of Bifurcations : |
518 |
| Number of Branches : |
1046 |
| Overall Width : |
6914.3 μm |
| Overall Height : |
2244.24 μm |
| Overall Depth : |
3862.05 μm |
| Average Diameter : |
2 μm |
| Total Length : |
150676 μm |
| Total Surface : |
946724 μm2 |
| Total Volume : |
473362 μm3 |
| Max Euclidean Distance : |
7792.77 μm |
| Max Path Distance : |
11968.8 μm |
| Max Branch Order : |
28 |
| Average Contraction : |
0.9 |
| Total Fragmentation : |
11698 |
| Partition Asymmetry : |
0.62 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
72.03° |
| Average Bifurcation Angle Remote : |
71.94° |
| Fractal Dimension : |
1.02 |
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