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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_146603 |
| Cell Name : | GFP_36_3 |
| Archive Name : | Firestein |
| Species Name : | human |
| Strain : | Not applicable |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | Not reported
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| Max Age : | Not reported
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| Gender : |
Not reported
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| Min Weight : | Not reported
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| Max Weight : | Not reported
|
| Development : | not reported |
| Primary Brain Region : | Not reported |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | induced pluripotent stem cell-(iPSC)-derived |
| Tertiary Cell Class : | microtubule-associated protein 2 (MAP2) positive |
| Original Format : | NeuronJ.swc |
| Experiment Protocol : | culture |
| Experimental Condition : | long isoform of Nitric Oxide Synthase 1 Adaptor Protein (NOS1AP-L) overexpression + D-serine treatment |
| Staining Method : | enhanced green fluorescent protein, immunostaining |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 20x |
| Reconstruction Method : | NeuronJ |
| Date of Deposition : | 2020-11-16 |
| Date of Upload : | 2021-01-05 |
| Persistence Vector : | GFP_36_3.pvec |
| Note : | We transfected human neurons with constructs encoding GFP fusions of NOS1AP-L on DD19. The hiPSC-derived neurons were treated on DD19 by adding vehicle to the medium containing dimethyl sulfoxide (DMSO, 0.01%). To maximally activate NMDAR glycine binding sites, neurons were treated for 48 h, from DD19-21, with 10 microM D-serine. Neuronal reconstructions were obtained at 21 days post-differentiation (DD21). |
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| Reference Article |
| Related Article Reference : | Characterization hiPSC-derived neural progenitor cells and neurons to investigate the role of NOS1AP isoforms in human neuron dendritogenesis. |
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| Measurements |
| Soma Surface : |
18.78 μm2 |
| Number of Stems : |
7 |
| Number of Bifurcations : |
7 |
| Number of Branches : |
21 |
| Overall Width : |
17.09 μm |
| Overall Height : |
26.92 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.3 μm |
| Total Length : |
90.28 μm |
| Total Surface : |
85.08 μm2 |
| Total Volume : |
6.38 μm3 |
| Max Euclidean Distance : |
22.83 μm |
| Max Path Distance : |
24.02 μm |
| Max Branch Order : |
2 |
| Average Contraction : |
0.9 |
| Total Fragmentation : |
155 |
| Partition Asymmetry : |
0.43 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
82.32° |
| Average Bifurcation Angle Remote : |
72.64° |
| Fractal Dimension : |
1.07 |
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