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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_177430 |
| Cell Name : | F31_neuron3_reg |
| Archive Name : | Guo |
| Species Name : | mouse |
| Strain : | C57BL/6J |
| Structural Domains : | Dendrites, Soma, Axon |
| Physical Integrity : | Dendrites & Axon Moderate |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 2.0 months
|
| Max Age : | 2.0 months
|
| Gender : |
Male
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | occipital |
| Tertiary Brain Region : | primary visual, layer 5 |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | pyramidal |
| Tertiary Cell Class : | pyramidal tract (PT) cell Sim1+ |
| Original Format : | Vaa3D.swc |
| Experiment Protocol : | in vivo |
| Experimental Condition : | Control |
| Staining Method : | AAV + eGFP |
| Slicing Direction : | horizontal |
| Slice Thickness : | 1
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x |
| Reconstruction Method : | Vaa3D |
| Date of Deposition : | 2021-11-16 |
| Date of Upload : | 2022-02-11 |
| Persistence Vector : | F31_neuron3_reg.pvec |
| Note : | To achieve sparse labeling of a few dozens of cortical neurons, a mixture of AAV2/1-hSyn-Cre and AAV2/1-CAG-Flex-EGFP was injected. The viral mixture was delivered through an oil hydraulic micromanipulator (Narishige, MO-10, 17 nL/min for 50 nL) into V1 (AP: -3.78, ML: 2.3, DV: 0.6). Each mouse was injected unilaterally and maintained for 5 weeks to allow stable and strong expression of EGFP. |
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| Reference Article |
| Related Article Reference : | Sparse imaging and reconstruction tomography for high-speed high-resolution whole-brain imaging |
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| Measurements |
| Soma Surface : |
187.24 μm2 |
| Number of Stems : |
8 |
| Number of Bifurcations : |
199 |
| Number of Branches : |
406 |
| Overall Width : |
4146.1 μm |
| Overall Height : |
4833.1 μm |
| Overall Depth : |
4233.09 μm |
| Average Diameter : |
2 μm |
| Total Length : |
51498.2 μm |
| Total Surface : |
323576 μm2 |
| Total Volume : |
161790 μm3 |
| Max Euclidean Distance : |
4875.58 μm |
| Max Path Distance : |
11067.4 μm |
| Max Branch Order : |
29 |
| Average Contraction : |
0.89 |
| Total Fragmentation : |
4452 |
| Partition Asymmetry : |
0.62 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
74.2° |
| Average Bifurcation Angle Remote : |
71.64° |
| Fractal Dimension : |
1.03 |
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