8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_151088
Cell Name :  DHHC9-GCP16-RasC181S9
Archive Name :  Shimell
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Dendrites, No Soma, No Axon
Physical Integrity :  Dendrites Moderate
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  18.0 days
Max Age :  18.0 days
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  embryonic
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Not reported
Original Format :  NeuronJ.ndf
Experiment Protocol :  culture
Experimental Condition :  Zdhhc9 + GCP16 + RasC181S
Staining Method :  enhanced green fluorescent protein
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  20x
Reconstruction Method :  NeuronJ
Date of Deposition :  2020-04-20
Date of Upload :  2021-02-25
Persistence Vector :  DHHC9-GCP16-RasC181S9.pvec
Note :  Hippocampal neurons were transfected at 10DIV with eGFP and Zdhhc9 shRNA plus its known cofactor GCP16 and the palmitoylation-deficient N-Ras (N-Ras C181SR; 1-3 mg of total plasmid DNA was added) and taken for analysis at 13 DIV.

Reference Article
Related Article Reference :  The X-Linked Intellectual Disability Gene Zdhhc9 Is Essential for Dendrite Outgrowth and Inhibitory Synapse Formation.

Measurements
Soma Surface :  N/A
Number of Stems :  1
Number of Bifurcations :  24
Number of Branches :  49
Overall Width :  323.49 μm
Overall Height :  324.3 μm
Overall Depth :  0 μm
Average Diameter :  0.25 μm
Total Length :  2340.8 μm
Total Surface :  1838.46 μm2
Total Volume :  114.9 μm3
Max Euclidean Distance :  336.36 μm
Max Path Distance :  898.69 μm
Max Branch Order :  21
Average Contraction :  0.97
Total Fragmentation :  688
Partition Asymmetry :  0.81
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  109.84°
Average Bifurcation Angle Remote :  106.87°
Fractal Dimension :  1.01

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