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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_151159 |
| Cell Name : | DHHC9-GCP16-Ras-shRNA7 |
| Archive Name : | Shimell |
| Species Name : | rat |
| Strain : | Sprague-Dawley |
| Structural Domains : | Dendrites, No Soma, No Axon |
| Physical Integrity : | Dendrites Moderate |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 18.0 days
|
| Max Age : | 18.0 days
|
| Gender : |
Male/Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | pyramidal |
| Tertiary Cell Class : | Not reported |
| Original Format : | NeuronJ.ndf |
| Experiment Protocol : | culture |
| Experimental Condition : | Zdhhc9 + GCP16 + Ras shRNA |
| Staining Method : | enhanced green fluorescent protein |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 20x |
| Reconstruction Method : | NeuronJ |
| Date of Deposition : | 2020-04-20 |
| Date of Upload : | 2021-02-25 |
| Persistence Vector : | DHHC9-GCP16-Ras-shRNA7.pvec |
| Note : | Hippocampal neurons were transfected at 10DIV with eGFP, Zdhhc9 shRNA plus its known cofactor GCP16, and Ras shRNA (1-3 mg of total plasmid DNA was added), and taken for analysis at 13 DIV. |
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| Reference Article |
| Related Article Reference : | The X-Linked Intellectual Disability Gene Zdhhc9 Is Essential for Dendrite Outgrowth and Inhibitory Synapse Formation. |
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| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
2 |
| Number of Bifurcations : |
12 |
| Number of Branches : |
26 |
| Overall Width : |
258.29 μm |
| Overall Height : |
415.72 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
2069.55 μm |
| Total Surface : |
1625.42 μm2 |
| Total Volume : |
101.59 μm3 |
| Max Euclidean Distance : |
304.61 μm |
| Max Path Distance : |
377.12 μm |
| Max Branch Order : |
9 |
| Average Contraction : |
0.97 |
| Total Fragmentation : |
584 |
| Partition Asymmetry : |
0.51 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
87.28° |
| Average Bifurcation Angle Remote : |
74.35° |
| Fractal Dimension : |
1.01 |
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0
