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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_141765 |
| Cell Name : | Cx35mut_L3_LC_7dpf_1uM-KN92 |
| Archive Name : | Sitaraman |
| Species Name : | zebrafish |
| Strain : | gjd2b(ncb215) |
| Structural Domains : | Dendrites, No Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 7.0 days
|
| Max Age : | 7.0 days
|
| Gender : |
Not reported
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| Min Weight : | Not reported
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| Max Weight : | Not reported
|
| Development : | larval |
| Primary Brain Region : | cerebellum |
| Secondary Brain Region : | cerebellar cortex |
| Tertiary Brain Region : | Purkinje layer |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | Purkinje |
| Tertiary Cell Class : | Not reported |
| Original Format : | Simple Neurite Tracer.traces |
| Experiment Protocol : | in vivo |
| Experimental Condition : | CaMKII inhibitor treatment |
| Staining Method : | green fluorescent protein |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 60x |
| Reconstruction Method : | Simple Neurite Tracer |
| Date of Deposition : | 2020-08-13 |
| Date of Upload : | 2020-10-22 |
| Persistence Vector : | Cx35mut_L3_LC_7dpf_1uM-KN92.pvec |
| Note : | This reconstruction belongs to an animal that was injected into single-celled zebrafish embryos at 0.05 pmoles per embryo with morpholino antisense oligonucleotides that mediates the knockdown of Cx35. The experimental treatment condition for this group includes CAMKII inhibitor treatment from 4 dpf till 7dpf with KN-93 (1 micromolar) and KN-92 (1 micromolar), and DMSO (1%). |
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| Reference Article |
| Related Article Reference : | Gjd2b-mediated gap junctions promote glutamatergic synapse formation and dendritic elaboration in Purkinje neurons |
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| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
1 |
| Number of Bifurcations : |
11 |
| Number of Branches : |
23 |
| Overall Width : |
5.44 μm |
| Overall Height : |
25.85 μm |
| Overall Depth : |
19.64 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
111.72 μm |
| Total Surface : |
87.75 μm2 |
| Total Volume : |
5.48 μm3 |
| Max Euclidean Distance : |
25.4 μm |
| Max Path Distance : |
49.75 μm |
| Max Branch Order : |
9 |
| Average Contraction : |
0.87 |
| Total Fragmentation : |
455 |
| Partition Asymmetry : |
0.64 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
104.09° |
| Average Bifurcation Angle Remote : |
106.08° |
| Fractal Dimension : |
1.09 |
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