|
| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_296915 |
| Cell Name : | Chuyu_Chen_Male-1979VH_1002-cell2 |
| Archive Name : | Chen_Parisiadou |
| Species Name : | mouse |
| Strain : | Tg(Adora2a-cre)KG139Gsat |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Incomplete |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 28.0 days
|
| Max Age : | 35.0 days
|
| Gender : |
Male
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | basal ganglia |
| Secondary Brain Region : | striatum |
| Tertiary Brain Region : | dorsal nucleus |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | medium spiny |
| Tertiary Cell Class : | indirect pathway, projection |
| Original Format : | Imaris.ims |
| Experiment Protocol : | in vivo |
| Experimental Condition : | haloperidol intraperitoneal injection |
| Staining Method : | green fluorescent protein, immunostaining |
| Slicing Direction : | coronal |
| Slice Thickness : | 30
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 60x |
| Reconstruction Method : | Imaris |
| Date of Deposition : | 2025-03-21 |
| Date of Upload : | 2025-05-01 |
| Persistence Vector : | Chuyu_Chen_Male-1979VH_1002-cell2.pvec |
| Note : | Only partial subtrees were reconstructed for this cell, hence the straight connections to the soma. P4-5-day-old Adora2aCre pups were cryoanesthetized and received ketoprofen for analgesia. They were placed on a cooling pad on a stereotaxic frame. 200 nl of the AAV-DIO-EGFP (RRID:Addgene_50457) virus were delivered into the dorsal striatum at a rate of 100 nl min-1 using Micro-4 controller (WPI). To ensure the dorsal striatum was targeted, the needle was placed 1 mm anterior to the midpoint between the ear and eye, 1.5 mm from the midline, and 1.8 mm ventral to the brain surface. Pups'' age and size determined slight adjustments in the coordinates. After the injection, the pups were warmed on a heating pad before returning to their home cages .P28-35 mice were administered haloperidol(1mg/kg) daily for 7 days. |
|
|
| Reference Article |
| Related Article Reference : | LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons. |
|
|
|
|
| Measurements |
| Soma Surface : |
1136.85 μm2 |
| Number of Stems : |
4 |
| Number of Bifurcations : |
451 |
| Number of Branches : |
906 |
| Overall Width : |
72.67 μm |
| Overall Height : |
92.35 μm |
| Overall Depth : |
39.56 μm |
| Average Diameter : |
0.51 μm |
| Total Length : |
1116.57 μm |
| Total Surface : |
1537.67 μm2 |
| Total Volume : |
196.85 μm3 |
| Max Euclidean Distance : |
72.86 μm |
| Max Path Distance : |
97.38 μm |
| Max Branch Order : |
62 |
| Average Contraction : |
0.96 |
| Total Fragmentation : |
3939 |
| Partition Asymmetry : |
0.79 |
| Average Rall's Ratio : |
1.49 |
| Average Bifurcation Angle Local : |
97.89° |
| Average Bifurcation Angle Remote : |
98.86° |
| Fractal Dimension : |
1.03 |
|
|
|
|
|
0
