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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_168372 |
| Cell Name : | ChiMERA_tamox-17 |
| Archive Name : | Soriano |
| Species Name : | mouse |
| Strain : | Mfn2-floxed UBC-CRE-ERT2 |
| Structural Domains : | Neurites, No Soma |
| Physical Integrity : | Neurites Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 17.5 days
|
| Max Age : | 17.5 days
|
| Gender : |
Male/Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | Not reported |
| Tertiary Cell Class : | Not reported |
| Original Format : | Simple Neurite Tracer.traces |
| Experiment Protocol : | culture |
| Experimental Condition : | Mfn2 knockout + Chimera Overexpression |
| Staining Method : | green fluorescent protein, Alexa Fluor 488, immunostaining |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 63x |
| Reconstruction Method : | Simple Neurite Tracer |
| Date of Deposition : | 2021-10-11 |
| Date of Upload : | 2021-11-19 |
| Persistence Vector : | ChiMERA_tamox-17.pvec |
| Note : | 4OH-Tamoxifen (1 micromolar) was added to the neuronal cultures at DIV3; neuritic length and complexity analysis was performed 72 h later. Mfn2 KO cells were transfected with plasmids encoding the artificial ER-mitochondria tether ChiMERA. |
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| Reference Article |
| Related Article Reference : | Mfn2 localization in the ER is necessary for its bioenergetic function and neuritic development. |
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| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
1 |
| Number of Bifurcations : |
16 |
| Number of Branches : |
33 |
| Overall Width : |
923.18 μm |
| Overall Height : |
192.2 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
2134.47 μm |
| Total Surface : |
1676.41 μm2 |
| Total Volume : |
104.78 μm3 |
| Max Euclidean Distance : |
547.08 μm |
| Max Path Distance : |
681.33 μm |
| Max Branch Order : |
9 |
| Average Contraction : |
0.95 |
| Total Fragmentation : |
1811 |
| Partition Asymmetry : |
0.56 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
94.34° |
| Average Bifurcation Angle Remote : |
87.74° |
| Fractal Dimension : |
1.02 |
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