8.6.96 - Released: 2025-11-05 - Content: 284158 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_156828
Cell Name :  ChAT-BF_L1_Projecting
Archive Name :  Fishell
Species Name :  mouse
Strain :  ChAT-FlpO; Rosa26Ai65
Structural Domains :  Dendrites, Soma, Axon
Physical Integrity :  Dendrites Moderate, Axon Complete
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  40.0 days
Max Age :  43.0 days
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  basal forebrain
Secondary Brain Region :  substantia innominata
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  cholinergic
Tertiary Cell Class :  projection, Somatosensory cortex targeting, L1-targeting
Original Format :  Neurolucida.dat
Experiment Protocol :  in vivo
Experimental Condition :  Control
Staining Method :  immunostaining
Slicing Direction :  coronal
Slice Thickness :  100  μm
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  20x
Reconstruction Method :  Neurolucida
Date of Deposition :  2021-05-24
Date of Upload :  2021-06-11
Persistence Vector :  ChAT-BF_L1_Projecting.pvec
Note :  Cav-2::Cre virus injections were performed on mice aged P30-P33. To limit the spread of viral particles allowing for layer- or pathway-specific injections, iontophoresis was used for all injections at 5 mA, 7 s on/3 s off, for a total of 10 min. The following stereotaxic coordinates were used: superficial layer S1 (AP: 1, ML: 3, DV: 0.15, 25 degrees tilt). Ten days following injection, mice were euthanized and perfused for analysis.

Reference Article
Related Article Reference :  Cellular birthdate predicts laminar and regional cholinergic projection topography in the forebrain.

Measurements
Soma Surface :  883.23 μm2
Number of Stems :  6
Number of Bifurcations :  439
Number of Branches :  884
Overall Width :  2834.34 μm
Overall Height :  5368.57 μm
Overall Depth :  4696.77 μm
Average Diameter :  1.18 μm
Total Length :  102373 μm
Total Surface :  377476 μm2
Total Volume :  154770 μm3
Max Euclidean Distance :  5250.7 μm
Max Path Distance :  13536 μm
Max Branch Order :  34
Average Contraction :  0.84
Total Fragmentation :  5656
Partition Asymmetry :  0.59
Average Rall's Ratio :  2.28
Average Bifurcation Angle Local :  77.77°
Average Bifurcation Angle Remote :  77.95°
Fractal Dimension :  1.04

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