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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_156827 |
| Cell Name : | ChAT-BF_Deep_Layer_Projecting |
| Archive Name : | Fishell |
| Species Name : | mouse |
| Strain : | ChAT-FlpO; Rosa26Ai65 |
| Structural Domains : | Dendrites, Soma, Axon |
| Physical Integrity : | Dendrites Moderate, Axon Complete |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 40.0 days
|
| Max Age : | 43.0 days
|
| Gender : |
Male/Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | basal forebrain |
| Secondary Brain Region : | substantia innominata |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | cholinergic |
| Tertiary Cell Class : | projection, Somatosensory cortex targeting, deep layer targeting |
| Original Format : | Neurolucida.dat |
| Experiment Protocol : | in vivo |
| Experimental Condition : | Control |
| Staining Method : | immunostaining |
| Slicing Direction : | coronal |
| Slice Thickness : | 100
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 20x |
| Reconstruction Method : | Neurolucida |
| Date of Deposition : | 2021-05-24 |
| Date of Upload : | 2021-06-11 |
| Persistence Vector : | ChAT-BF_Deep_Layer_Projecting.pvec |
| Note : | Cav-2::Cre virus injections were performed on mice aged P30-P33. To limit the spread of viral particles allowing for layer- or pathway-specific injections, iontophoresis was used for all injections at 5 mA, 7 s on/3 s off, for a total of 10 min. The following stereotaxic coordinates were used: deep layer S1 (AP: 1, ML: 3, DV: 0.85). Ten days following injection, mice were euthanized and perfused for analysis. |
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| Reference Article |
| Related Article Reference : | Cellular birthdate predicts laminar and regional cholinergic projection topography in the forebrain. |
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| Measurements |
| Soma Surface : |
613.27 μm2 |
| Number of Stems : |
3 |
| Number of Bifurcations : |
365 |
| Number of Branches : |
733 |
| Overall Width : |
2052.2 μm |
| Overall Height : |
2883.39 μm |
| Overall Depth : |
893.46 μm |
| Average Diameter : |
2.16 μm |
| Total Length : |
55280.2 μm |
| Total Surface : |
379091 μm2 |
| Total Volume : |
242916 μm3 |
| Max Euclidean Distance : |
2991.94 μm |
| Max Path Distance : |
8930.72 μm |
| Max Branch Order : |
60 |
| Average Contraction : |
0.86 |
| Total Fragmentation : |
4325 |
| Partition Asymmetry : |
0.68 |
| Average Rall's Ratio : |
2.4 |
| Average Bifurcation Angle Local : |
91.03° |
| Average Bifurcation Angle Remote : |
93.28° |
| Fractal Dimension : |
1.04 |
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