|
| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_160925 |
| Cell Name : | Casp6_WT_Neuron_2 |
| Archive Name : | Sjostrom |
| Species Name : | mouse |
| Strain : | C57BL/6 |
| Structural Domains : | Dendrites, Soma, Axon |
| Physical Integrity : | Dendrites & Axon Moderate |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 11.0 days
|
| Max Age : | 16.0 days
|
| Gender : |
Male/Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | young |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | CA1 |
| Tertiary Brain Region : | pyramidal layer |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | pyramidal |
| Tertiary Cell Class : | Not reported |
| Original Format : | Neuromantic.swc |
| Experiment Protocol : | in vitro |
| Experimental Condition : | recombinant human caspase-6 WT |
| Staining Method : | Alexa Fluor 594 |
| Slicing Direction : | custom |
| Slice Thickness : | 300
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | water |
| Magnification : | 40x |
| Reconstruction Method : | Neuromantic |
| Date of Deposition : | 2021-07-20 |
| Date of Upload : | 2021-07-30 |
| Persistence Vector : | Casp6_WT_Neuron_2.pvec |
| Note : | Recombinant human caspase-6 WT was activated in Stennicke buffer at 37 Celsius degrees for 15 min and diluted to 10 pg/10 microL in internal solution with 30-60 micromolar Alexa 594 or 40-80 micromolar Alexa 488, and osmolarity double checked to be around 310 mOsm. |
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| Reference Article |
| Related Article Reference : | Rare CASP6N73T variant associated with hippocampal volume exhibits decreased proteolytic activity, synaptic transmission defect, and neurodegeneration. |
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|
| Measurements |
| Soma Surface : |
423.25 μm2 |
| Number of Stems : |
8 |
| Number of Bifurcations : |
60 |
| Number of Branches : |
128 |
| Overall Width : |
415.62 μm |
| Overall Height : |
919.84 μm |
| Overall Depth : |
82.25 μm |
| Average Diameter : |
3.39 μm |
| Total Length : |
10006.8 μm |
| Total Surface : |
108042 μm2 |
| Total Volume : |
102222 μm3 |
| Max Euclidean Distance : |
769.65 μm |
| Max Path Distance : |
831.66 μm |
| Max Branch Order : |
16 |
| Average Contraction : |
0.95 |
| Total Fragmentation : |
1768 |
| Partition Asymmetry : |
0.51 |
| Average Rall's Ratio : |
1.93 |
| Average Bifurcation Angle Local : |
62.96° |
| Average Bifurcation Angle Remote : |
51.38° |
| Fractal Dimension : |
1.01 |
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